Abstract: Pot experiments were conducted to investigate the cultivar difference of Fe, Mn, Cu and Zn concentration and their types in triticale grain in response to nitrogen application. 31 triticale varieties with different inheritance background were used as experimental materials. Low and normal content nitrogen were supplied. The results showed that: 1) Under normal nitrogen supply, Fe and Zn content in triticale grain were significant higher than that of low nitrogen supply. The differences of Mn and Cu content under two nitrogen levels were not significant. Significant difference of Fe, Mn, Cu and Zn contents in triticale grain were observed under the same nitrogen levels. The coefficient of variation was from 15.07 % to 38.69 %. 2) The sensitivity discrepancy in view of Fe, Mn, Cu and Zn contents existed in the grain of triticale varieties under nitrogen supply. Insensitive type, intermediate type and sensitive type were classified based on sensitivity discrepancy of Fe, Mn, Cu and Zn contents under nitrogen supply. 3) According to correlation analysis, no positive significant correlation was observed between Fe and Mn content and1000-grain weight. The results showed a highly significant positive correlation between the content of Cu, Zn and1000-grain weight (the correlation coefficient were 0.45 and 0.44, respectively). The content of Fe, Mn, Cu and Zn had a highly significant positive correlation to nitrogen content in triticale grain (the correlation coefficient were 0.34, 0.55, 0.47 and 0.71, respectively). The conclusion could be used as a reference for excavation and application of advantaged gene in triticale.