耐盐和非耐盐大麦幼苗叶片抗氧化及抗坏血酸–谷胱甘肽循环系统对 NaCl 胁迫的反应差异

李琲琲; 刘志萍; 张凤英; 包海柱; 孟繁昊; 王聪; 杨恒山; 徐寿军

doi:10.11674/zwyf.16315

耐盐和非耐盐大麦幼苗叶片抗氧化及抗坏血酸–谷胱甘肽循环系统对 NaCl 胁迫的反应差异

Response difference of antioxidant and ascorbate-glutathione cycle system to NaCl stress between salt tolerant and sensitive barley cultivars at the seedling stage

摘要

摘要:
目的研究 NaCl 胁迫下，耐盐和非耐盐品系大麦幼苗叶片抗氧化系统及抗坏血酸–谷胱甘肽循环的反应差异。
方法以耐盐品系 12pj-118 和非耐盐品系 12pj-045 为材料进行了水培试验。营养液中设定了 6 个 NaCl 浓度：0、100、200、300、400、500 mmol/L。在大麦苗生长至 3 叶 1 心时，取样分析测定叶片中活性氧代谢、抗氧化酶活性以及抗坏血酸–谷胱甘肽循环变化。
结果随着 NaCl 胁迫的增加，2 个品系的 \rmO_\small 2^\overline \,\cdot\, 产生速率、H₂O₂ 含量和 MDA 含量均逐渐增加，耐盐品系 12pj-118 的增幅均小于非耐盐品系 12pj-045；SOD、POD、CAT、APX、GR 活性、AsA 含量、GSH 含量和 AsA/DHA 比值均呈先上升后下降的趋势。12pj-118 的 SOD、POD、CAT 活性在各 NaCl 浓度胁迫下的增幅大于 12pj-045，降幅小于 12pj-045；12pj-118 的 APX、GR 活性在同一盐浓度胁迫下的增幅均大于非耐盐品系 12pj-045，降幅小于 12pj-045；在各 NaCl 浓度下，12pj-118 的 AsA 含量和 AsA/DHA 比值较对照增幅均大于 12pj-045；GSH/GSSG 比值呈波状变化，12pj-118 在较高 NaCl 浓度下，仍能够维持较高的 GSH 含量和 GSH/GSSG 比值。显示 12pj-118 较 12pj-045 有较强的耐盐性。
结论耐盐和非耐盐品系大麦叶片抗氧化及抗坏血酸–谷胱甘肽循环系统在 NaCl 胁迫下的反应不同。在一定范围内，随着盐胁迫增强，耐盐品系 12pj-118 叶片 SOD、POD、CAT、APX 和 GR 活性、AsA 和 GSH 含量增幅均大于非耐盐品系 12pj-045，降幅小于 12pj-045，表明叶片抗氧化及抗坏血酸–谷胱甘肽循环系统与大麦幼苗抗盐性密切相关。

Abstract:
ObjectivesThe aim of this study was to compare the response differences of antioxidant and ascorbate-glutathione cycle system to NaCl stress between the salt tolerant and sensitive barley cultivars at the seedling stage.
MethodsA hydroponic experiment was conducted using salt-tolerant cultivar (12pj-118) and non-tolerant cultivar (12pj-045) of barley as materials. Six NaCl stress concentrations were set up, including 0, 100, 200, 300, 400 and 500 mmol/L. The plant samples were collected when the forth leaf emerged. The active oxygen metabolism, antioxidant enzyme activity and ascorbate glutathione cycle were determined.
ResultsThe results showed that the \rmO_\small 2^\overline \,\cdot\, production rate, H₂O₂ content and MDA content were increased gradually with the increase of NaCl levels, and the increased rates of the 12pj-118 were lower than those of the 12pj-045. The activities of SOD, POD, CAT, GR and APX, the contents of AsA and GSH and the ratios of AsA/DHA were increased at first and then decreased. The increased rates of the SOD, POD and CAT activities were much higher in the 12pj-118 under different NaCl concentrations at first, and reduced rates were lower later. The increased rates of APX and GR activities of 12pj-118 were higher than those of 12pj-045 under the same salt concentration, and the reduced rates were lower. Under different NaCl concentrations, the increased rates of the AsA contents and AsA/DHA ratios of 12pj-118 were higher than those of 12pj-045. The ratios of GSH/GSSG showed a wavy change in two barley cultivars, and the 12pj-118 was able to maintain high GSH contents and GSH/GSSG ratios under higher NaCl concentrations. These results indicated that the salt resistance of 12pj-118 was better than the 12pj-045.
ConclusionsThe response of NaCl stress was different on antioxidant system and ascorbate-glutathione cycle at the seedling stage. At a given range, with the increase of NaCl concentration, the increased rates of the SOD, POD, CAT, APX, GR, AsA and GSH of the12pj-118 were much higher than those of the 12pj-045 under different NaCl concentrations, and reduced rates were lower. The results indicated that antioxidant activity and ascorbate glutathione cycle were closely related to salt tolerance of barley seedlings.

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