Abstract:
Objectives Nucleoside component is one of the main active substances of Fritillaria. The contribution rate of Zn, B and Mo application on the content of nucleosides in Fritillaria was studied for the optimization of their application formula of Fritillaria unibracteata var. wabuensis.
Methods A plot experiment was conducted at the standardized production base of Sichuan Fritillaria. The F. unibracteata var. wabuensis bulbs of four-year-old and 12 mm in size were used as materials. A five-level quadratic orthogonal rotation method was used for the treatment design of the experiment. The 20%, 30%, 30% and 20% of the total Zn, B and Mo fertilizers were foliar applied at the budding, early flowering, full flowering and young fruit stages of Fritillaria, respectively. The Fritillaria bulbs were harvested from the experimental plots, and they were used to determine the content of 10 nucleosides.
Results The least square method, gray correlation degree and DTOPSIS method were used to calculate the contribution rates of trace elements and nucleosides, the influence of the single factors and interaction of two factors on the nucleoside components to obtain the optimal fertilization recommendation.
Results By contribution rate analysis, zinc fertilizer contributed the most to uridine, thymidine and inosine, boron fertilizer contributed the most to cytidine and guanosine, and molybdenum contributed the most to uracil and thymidine. Single factor analysis showed that zinc fertilizer and boron fertilizer had significant effects on cytidine, and molybdenum fertilizer had significant effect on thymidine. Interaction analysis showed that zinc-boron interaction synergistically exerted uracil, but antagonized thymine, uridine and thymidine; zinc-molybdenum interaction synergized with uracil, uridine and inosine; boron-molybdenum interaction antagonized cytidine, thymidine, guanosine and uridine. The grey correlation degree and DTOPSIS method analysis showed the comprehensive optimal treatment.
Conclusions The optimal fertilization was determined as zinc fertilizer 8.05–11.85 kg/hm2; boron fertilizer 11.70–16.47 kg/hm2; and molybdenum fertilizer 0.36–0.51 kg/hm2. By using grey correlation and DTOPSIS method, when the zinc fertilizer was 15.71 kg/hm2, boron fertilizer 5.53 kg/hm2 and molybdenum fertilizer 0.65 kg/hm2, the content of 10 nucleosides was optimal.