• ISSN 1008-505X
  • CN 11-3996/S
贾春花, 张林琳, 张民, 刘之广, 郑磊, 孙玲丽, 孙正. 小肽螯合铁缓解樱桃萝卜苗期高温胁迫的生理机制[J]. 植物营养与肥料学报, 2019, 25(8): 1393-1400. DOI: 10.11674/zwyf.18343
引用本文: 贾春花, 张林琳, 张民, 刘之广, 郑磊, 孙玲丽, 孙正. 小肽螯合铁缓解樱桃萝卜苗期高温胁迫的生理机制[J]. 植物营养与肥料学报, 2019, 25(8): 1393-1400. DOI: 10.11674/zwyf.18343
JIA Chun-hua, ZHANG Lin-lin, ZHANG Min, LIU Zhi-guang, ZHENG Lei, SUN Ling-li, SUN Zheng. Physiological mechanism of mitigation of high temperature stress by peptide-chelated iron application in cherry radish seedling[J]. Journal of Plant Nutrition and Fertilizers, 2019, 25(8): 1393-1400. DOI: 10.11674/zwyf.18343
Citation: JIA Chun-hua, ZHANG Lin-lin, ZHANG Min, LIU Zhi-guang, ZHENG Lei, SUN Ling-li, SUN Zheng. Physiological mechanism of mitigation of high temperature stress by peptide-chelated iron application in cherry radish seedling[J]. Journal of Plant Nutrition and Fertilizers, 2019, 25(8): 1393-1400. DOI: 10.11674/zwyf.18343

小肽螯合铁缓解樱桃萝卜苗期高温胁迫的生理机制

Physiological mechanism of mitigation of high temperature stress by peptide-chelated iron application in cherry radish seedling

  • 摘要:
    目的 樱桃萝卜 (Raphanus sativus L.var.radculus Pers.) 易受高温胁迫引起的活性氧爆发导致损伤,进而影响生长。小肽螯合铁是一种外源生物刺激素,可显著提高作物抗逆性。本文探讨了不同小肽螯合铁处理浓度对樱桃萝卜中与高温胁迫相关的生理活性成分的影响,为利用小肽螯合铁减少高温胁迫对作物生长的危害提供理论依据。
    方法 以樱桃萝卜为试材进行浸种和灌根试验。试验设常温25℃不加生物刺激素的清水处理 (CK0);胁迫温度为30℃下不加生物刺激素的清水处理 (CK),浓度为66.7 mg/L的芸苔素内酯处理 (BHG)以及21.0、32.0、48.0 mg/L浓度的小肽螯合铁处理 (T-Fe-1、T-Fe-2、T-Fe-3) ,共6个处理。在樱桃萝卜生长28天后,采集植株样,称重,并测定内源激素含量和抗氧化及光合系统酶活性。
    结果 高温胁迫下,与清水处理比较,小肽螯合铁浓度为21.0 mg/L时显著提高了樱桃萝卜的生长素 (IAA) 水平144.2%,显著提高整株生物量26.0%、根系长度35.8%;显著提高脯氨酸 (Pro) 含量229.9%;显著提高抗氧化系统中抗坏血酸过氧化物酶 (APX) 活性21.7%、谷胱甘肽还原酶 (GR) 活性11.7%,然而对超氧阴离子 (\rmO_\small 2^\overline \,\cdot\,) 活性、丙二醛 (MDA) 含量和净光合速率 (Pn) 影响不显著。高温胁迫条件下,与促生效果较好的66.7 mg/L芸苔素内酯处理比较,21.0 mg/L小肽螯合铁可显著提高IAA含量141%,提高Pro含量11.4%,并分别显著提高过氧化氢酶 (CAT)、APX和GR活性262.5%、44.7%和19.4%,从而显著降低\rmO_\small 2^\overline \,\cdot\,活性18.6%。高温胁迫条件下,32.0 mg/L小肽螯合铁较清水处理可显著提高Pro含量184%,分别提高超氧化物歧化酶 (SOD) 和GR活性 366%和19.8%,并分别降低过氧化氢 (H2O2)、\rmO_\small 2^\overline \,\cdot\,活性和MDA含量41.5%、24.2%和76.5%,但其整株生物量显著降低36.5%;而48.0 mg/L小肽螯合铁处理较清水处理可显著提高IAA、Pro含量26.1%和132.6%,SOD和GR活性分别显著提高633.4%和19.4%,显著降低H2O2\rmO_\small 2^\overline \,\cdot\,活性29.9%和26.2%,整株生物量无显著差异,促生效果不明显。
    结论 本试验条件下,施用21.0 mg/L小肽螯合铁可显著提高樱桃萝卜内源激素和脯氨酸水平、抗氧化系统活性,从而显著缓解高温胁迫损伤,提高生物产量。超过该剂量施用小肽螯合铁有抑制作物生长的风险。

     

    Abstract:
    Objectives Cherry radish (Raphanus sativus L.var. radculus Pers.) is vulnerable to high temperature induced damages. Peptide-chelated iron (T-Fe) is effective in improving the crop’s resistance and alleviating damages caused by high temperature stress as an exogenous biostimulant.The effect of different T-Fe treatment concentrations on the high temperature stress related physiological regulators was studied, which would provide support for the effective application of T-Fe in reducing the hot damage to crop.
    Methods The seed soaking and irrigation experiment was conducted using cherry radish as the test crop. For the experiments, 25℃ and 30℃ without any biostimulants were used as the controls (CK0, CK), the brassin-heteroauxin-gibberellic acid treatment with concentration of 66.7 mg/L (BHG), the peptide-chelated iron treatments with 21.0, 32.0 and 48.0 mg/L (T-Fe-1, T-Fe-2, T-Fe-3) at 30℃ were set up. The plant biomass were weighed, and the content of auxin (IAA), abscisic acid (ABA) and proline (Pro), and the activity of superoxide dismutase (SOD), ascorbate peroxidase (APX) and glutathione reductase (GR), net photosynthetic rate (Pn) and transpiration rate (Tr) were measured after the cherry radish seedling grown for 28 days.
    Results Comparing with CK, the IAA level of cherry radish in T-Fe-1 treatment was significantly increased by 144.2%, the biomass was increased by 26.0% and root length increased by 35.8%. The content of Pro was significantly increased by 229.9%. Meanwhile, APX activity was significantly increased by 21.7% in the antioxidant system, GR activity was significantly increased by 11.7%. There was no significant difference in superoxide anion (\rmO_\small 2^\overline \,\cdot\, ) activity, malondialdehyde (MDA) content and Pn. Compared with BHG treatment, the content of IAA was significantly increased by 141%, Pro content increased by 11.4%, the peroxidase (CAT), APX and GR activities were significantly increased by 262%, 44.7% and 19.4%, respectively, the \rmO_\small 2^\overline \,\cdot\, content was significantly reduced by18.6%. In the T-Fe-2 treatment, comparing with CK, the Pro content was significantly increased by 184%, SOD activity and GR activity were significantly increased by 366% and 19.8%, respectively, thus significantly reducing hydrogen peroxide (H2O2), \rmO_\small 2^\overline \,\cdot\, activity, MDA content by 41.5%, 24.2% and 76.5%, respectively, but the biomass was significantly reduced by 36.5%. In the T-Fe-3 treatment, comparing with CK, the IAA and Pro contents were significantly increased by 26.1% and 132.6%, the SOD and GR activities were significantly increased by 633.4%and 19.4%, respectively, the H2O2 and \rmO_\small 2^\overline \,\cdot\, activity were significantly reduced by 29.9% and 26.2%, respectively, there was no significant difference in total biomass, and the growth-promoting effect was not obvious.
    Conclusions Under the experimental conditions, applying peptide-chelated iron in concentration of 21 mg/L is satisfactory in the promotion effect of plant endogenous hormone levels and proline and activity of the antioxidant system of cherry radish, which consequently alleviate the damage caused by high temperature stress significantly. The concentration higher than 21 mg/L might cause inhibition of crop growth.

     

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