• ISSN 1008-505X
  • CN 11-3996/S
刘文莉, 张崇邦, 刘亮, 管铭, 王江. 氨氧化基因丰度和潜在氨氧化速率对加拿大一枝黄花入侵和土壤类型的响应[J]. 植物营养与肥料学报, 2020, 26(7): 1367-1374. DOI: 10.11674/zwyf.19364
引用本文: 刘文莉, 张崇邦, 刘亮, 管铭, 王江. 氨氧化基因丰度和潜在氨氧化速率对加拿大一枝黄花入侵和土壤类型的响应[J]. 植物营养与肥料学报, 2020, 26(7): 1367-1374. DOI: 10.11674/zwyf.19364
LIU Wen-li, ZHANG Chong-bang, LIU Liang, GUAN Ming, WANG Jiang. Responses of soil ammonia-oxidizing gene abundances and potential ammonia-oxidizing rate to Solidago canadensis invasion and soil types[J]. Journal of Plant Nutrition and Fertilizers, 2020, 26(7): 1367-1374. DOI: 10.11674/zwyf.19364
Citation: LIU Wen-li, ZHANG Chong-bang, LIU Liang, GUAN Ming, WANG Jiang. Responses of soil ammonia-oxidizing gene abundances and potential ammonia-oxidizing rate to Solidago canadensis invasion and soil types[J]. Journal of Plant Nutrition and Fertilizers, 2020, 26(7): 1367-1374. DOI: 10.11674/zwyf.19364

氨氧化基因丰度和潜在氨氧化速率对加拿大一枝黄花入侵和土壤类型的响应

Responses of soil ammonia-oxidizing gene abundances and potential ammonia-oxidizing rate to Solidago canadensis invasion and soil types

  • 摘要:
    目的 土壤类型和植物入侵影响土壤微生物群落结构和功能,基于此,我们研究加拿大一枝黄花 (Solidago canadensis) 入侵两种类型土壤后,土壤中氨氧化古细菌 (AOA) 和氨氧化细菌 (AOB) 的基因丰度和潜在氨氧化速率 (PAOR) 的变化规律及影响机理。
    方法 云南和浙江是加拿大一枝黄花入侵的重点地区,本研究在云南省滇池周边的海东湿地公园、捞鱼河湿地公园和安乐村 (土壤类型均为冲积土) 以及浙江省东部的杭州湾湿地公园、临海上盘镇和路桥镇峰江村 (土壤类型均为黄泥田土),分别选择一块采样地,面积为100~150 m2。在每块采样地加拿大一枝黄花入侵 (Mono) 和未入侵 (Nat) 之处,划出0.5 m × 0.5 m,采集土壤和植物样品。Mono和Nat地块相距10~20 m,每个地块重复3次。采用qPCR和室内培养技术分析土壤中AOA、AOB基因丰度和PAOR,用植物生态和土壤化学方法分析植物生物量和土壤化学性状。
    结果 加拿大一枝黄花入侵后,冲积土中AOA丰度和PAOR显著下降,黄泥田土中AOA丰度和PAOR却显著提高,而AOB丰度在两种类型土壤中均显著提高。Nat冲积土中AOA丰度和PAOR大于黄泥田土,而Mono条件下冲积土中AOA丰度和PAOR小于黄泥田土,AOB丰度在两种土壤类型中均变化较小。Mono样地植被地上部分和地下部分生物量、土壤有机质含量和pH是影响冲积土和黄泥田土AOA丰度和PAOR的重要因素。与AOA不同,冲积土AOB丰度仅受植物地下部分生物量的影响,而黄泥田土AOB丰度同时受植物地下部分生物量、土壤总磷含量和pH影响。
    结论 加拿大一枝黄花入侵大大增加了植被地上部和地下部生物量,进而为微生物提供了大量的碳源,同时提高了土壤pH,因此,氨氧化细菌AOB的丰度显著增加。土壤类型仅影响AOA的丰度,对AOB和氨氧化潜力没有显著影响,而加拿大一枝黄花入侵显著影响两类土壤中的AOA、AOB丰度以及氨氧化潜力。

     

    Abstract:
    Objectives Soil type and plant invasion are two important factors in shaping soil microbial community structure and function. Based on this, we studied the variation in gene abundances of soil ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) and the potential ammonia-oxidizing rate (PAOR), as well as the related mechanisms caused by Solidago canadensis invasion.
    Methods Both Yunnan and Zhejiang Province have been heavily invaded by S. canadensis since 2010. The soil sampling areas were chosen nearby Dianchi of Yunnan Province and along the coastline of Eastern Zhejiang Province, covered 100–150 m2 each area. In Yunnan, the three plots located in Haidong wetland park, Laoyu river wetland park and Anle village, where was alluvial soil. In Zhejiang, the three plots were in Hangzhou bay wetland park, Shangpan town of Linhai and Fengjiang village of Luqiao, where was yellow mud soil. In each plot, selecting three points of 0.5 m × 0.5 m with invasion of S. canadensis (Mono) and three points without (Nat). Plant and soil samples were collected and analyzed.
    Results Under S. canadensis invasion, the AOA abundance and PAOR decreased in the alluvial soil but increased in the yellow mud soil, while the AOB abundances increased in both soil types. Under the native vegetation, both the AOA abundance and PARO in the alluvial soil were greater than those in yellow mud soil, while under S. canadensis vegetation, they were opposite. The AOB abundance in two soil types did not significantly changed no matter invaded by S. canadensis or not. In S. canadensis invaded point, the mediating factors for AOA abundance and PAOR were the above- and under-ground plant biomasses, soil organic matter content and pH, and that for AOB abundance in the alluvial soil was only underground plant biomass, and in yellow mud soil were underground plant biomass, total P content and pH.
    Conclusions S. canadensis has much higher above- and under-ground biomass than native plants, the evasion of S. canadensis provides much more organic carbon source and leads to increased soil pH, therefore, S. canadensis invasion obviously influences AOA, AOB abundance and the potential ammonia oxidation rate in two soil types. Soil type only affects AOA abundance, but had no significant effect on AOB abundance and the potential ammonia oxidation rate.

     

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