Abstract: This paper studied the effects of application N, Zn and Mn in a state of balance and Cl of contain Cl nitrogen fertilizer (NH4Cl) on the nutrition quality of crude potein and amino acid in winter wheat grain by field experiment and pot experiment. The results showed that the content of crude protein and all kinds of amino acid in wheat grain were increased by N applied, but the rate of the total essential amino acid in the protein were decreased. Application Zn with appropriate quantity could increase the content of crude protein and essential amino acid and raise the nutrition value of wheat grain. The content of protein and total amino acid could not be increased. but the content of the essential amino acid was increased by Mn applied. Application Mn could improve the quality of wheat grain. There were possitive combined effect between N-Zn, N-Mn, Zn-Mn combined applicatiaon and the content and quality of wheat grain.Combined application of N, Zn, Mn could not only increase the content of protein and total amino acid, but also raise the rate of the total essential amino acid in protein and improve the nutrition quality of wheat grain. The content of protein and amino acid in wheat grain could be increased and its nutrition value was not influence by application Cl with appropriate quantity.