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汪洪, 褚天铎. 缺镁对菜豆幼苗膜脂过氧化及体内活性氧清除酶系统的影响[J]. 植物营养与肥料学报, 1998, 4(4): 386-392. DOI: 10.11674/zwyf.1998.0410
引用本文: 汪洪, 褚天铎. 缺镁对菜豆幼苗膜脂过氧化及体内活性氧清除酶系统的影响[J]. 植物营养与肥料学报, 1998, 4(4): 386-392. DOI: 10.11674/zwyf.1998.0410
Wang Hong, Chu Tianduo. EFFECT OF Mg DEFICIENCY ON PLASMA MEMBRANE PERMEABILITY, MEMBRANE LIPID PEROXIDATION AND ENDOGENOUS OXYGEN RADICALS SCAVENGING ENZYMES SYSTEMS IN COMMON BEAN[J]. Journal of Plant Nutrition and Fertilizers, 1998, 4(4): 386-392. DOI: 10.11674/zwyf.1998.0410
Citation: Wang Hong, Chu Tianduo. EFFECT OF Mg DEFICIENCY ON PLASMA MEMBRANE PERMEABILITY, MEMBRANE LIPID PEROXIDATION AND ENDOGENOUS OXYGEN RADICALS SCAVENGING ENZYMES SYSTEMS IN COMMON BEAN[J]. Journal of Plant Nutrition and Fertilizers, 1998, 4(4): 386-392. DOI: 10.11674/zwyf.1998.0410

缺镁对菜豆幼苗膜脂过氧化及体内活性氧清除酶系统的影响

EFFECT OF Mg DEFICIENCY ON PLASMA MEMBRANE PERMEABILITY, MEMBRANE LIPID PEROXIDATION AND ENDOGENOUS OXYGEN RADICALS SCAVENGING ENZYMES SYSTEMS IN COMMON BEAN

  • 摘要: 营养液中培养菜豆幼苗,缺镁菜豆植株生长受抑,生物量显著低于正常供应镁的植株,体内镁浓度和镁的积累总量均较低。严重缺镁黄化叶片中叶绿素含量下降。完整叶绿体的希尔反应值在缺镁与正常植株间无差别。缺镁菜豆植株的根和叶膜造性(以相对电导率表示)增加,叶片中丙二醛含量升高。活性氧清除酶系统:SOD、POD、CAT活性在缺镁菜豆真叶中高于正常植株,老叶中抗坏血醒过氧化物酶(AsA-POD)活性增加程度也较大。

     

    Abstract: Common bean Phaseolus vulgaris L. plants were cultured in solution with Mg deficient (0 mmol/L) and nirmal Mg supply 1. 2mmol/L. Plants under Mg deficiency showed intervenial chlorosis, necrosis in primary leaves and first trifoliate leaves. Normal Mg supply increased plants dry matter weight and concentration of Mg in leaves, stems, and roots. There was little difference of concentration of chlorophyll between those leaves without yellow and chlorosls under Mg deficiency and normal leaves with Mg sufficiency, but concentration of chlorophyll in yellowish leaves from Mg deficiency was lower. Hill reaction values of intact chloroplasts in Mg deficient leaves were similar to those in Mg sufficient lesves. Mg deficiency caused the increase of relative electric conductance in extracting solution of lesves and roots. Concentration of malondialdehyde also remained pronounced levels in leaves. Comparing to Mg sufficient leaves, the activies of superoxide dismutase (SOD, EC 1. 15. 11), ascorbate peroxidase AsA-POD, EC 1. 11. 17, guaiacol peroxidase (EC1.11. 17) and catalase(CAT. EC L 11. 16) were significantly enhanced inthe leaves by Mg deficiency. It seemed that Mg deficiency affected plasma membrane permeability, membrane llpld peroxldatl0n and endogenous oxygen radicals scavenglng enzymes Systems in bean seedlings.

     

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