Abstract: Using BIOLOG method, the microbial community and functional diversity around circumference of cucumber seeds of four different cucumber species were studied during different germinating phases of 12h, 36h, and 72h .Seed were cultured in pots which were placed in light-emitted feeding box .The microorganisms around circumference of cucumber seeds were cultured in GN plate with 96 small rooms and the OD value was determined at different culture time.The results showed that characteristics of carbon metabolism were various among different cucumber species with the germinating time of 12h and 36h, so were the different treatments on the same species .Regarding the C metabolic forms, the utilization rate of saccharide was the highest (OD >1.4) , while the utilization rate of amino-acid was the lowest ( OD <0.8) , and the organic acid was intermediate .During the time of seed germination, the evolvement characters of domi- nant microbial community in term of C source metabolism were :amino acid community ※organic acid community ※sugar metabolism community, which was the dormant one ultimately .