Abstract: Banana fusarium-wilt disease is one of the most important soil-borne diseases which severely inhibit the development of banana production industries both in China and in the world. Biocontrols of the banana fusarium-wilt disease are proved economical and environmentally friendly. A pot experiment was carried out to investigate effects of a new bioorganic fertilizer(BIO)on banana plant growth and the suppression on the disease. The results are as follows: 1)45 days after transplantation of banana seedlings, fresh weight, dry weight, plant height and stem girth of the plants in the treatments of BIO fertilization are increased by 31.9%-93.8%, 5.9%-43.1%, 43.9%-62.9% and 12.3%-18.5%, respectively compared with the control. 2)When wilt disease just occurs, chitinase activities in the treatments of BIO fertilization are 2.64-12.88 units higher than that of the control; β-1,3-glucanase activities in the treatments of nursery applications of BIO are increased by 5.22 or 7.22 times than that without nursery application of BIO; and the concentrations of callose in root tips and stem bases of the treatments of BIO fertilization are decreased by 59.7%-77.9% and 47.6%-68.9%, respectively as compared with the control. 3)In contrast to the control, numbers of bacteria and actinomycetes in rhizosphere of the treatment of BIOns+BIOts are increased by 1.56-1.76 times and 1.34-1, 50 times, respectively，while numbers of fungi and pathogen are decreased by 19.2%-29.6% and 38.0%-63.9%, respectively. 4)Compared to the control, the banana fusarium-wilt disease indices of BIOns+BIOts are below 2.4, which are reduced by 47.8%-56.5%. These results suggest that the way of BIO application both in nursery pots and in transplanted soils can effectively overcome the occurrence of fusarium wilt disease of banana.