Abstract: 【Objectives】 The experimental objective is to investigate the differences in N uptake and utilization in the machine-transplanted rice genotypes and explore an effective pathway improving uptake and utilization of N in the machine-transplanted rice genotypes.【Methods】 A field experiment was designed in randomized blocks to determine the N content, yields, the characteristics of N accumulation and dry matter weight, distribution and translocation of N in the different machine-transplanted rice genotypes at the growth stages of three medium-maturing varieties, three late-maturing varieties and four japonica rice cultivars, and explain the reasons causing the differences in the characteristics mentioned above.【Results】 1) The accumulation dynamics of N in the machine-transplanted rice was exhibited by a logistic curve. 2) N content in the machine-transplanted rice decreased through the growth period. The content of N in japonica rice cultivars was higher than in indica hybrid rice cultivars during jointing-heading, then that was gradually decreased and became lower than in indica hybrid rice cultivars at maturity. The content of N in medium-maturing indica hybrid rice cultivars reached the highest value at maturity stage. 3) The ultimate N uptake in the japonica rice cultivars was 23.0% lower than in medium-maturing indica hybrid rice cultivars, and 33.1% lower than in late-maturing indica hybrid rice cultivars. 4) Medium-maturing indica hybrid rice cultivars exhibited the highest N accumulation during heading-maturity, and a highly N distribution ratio in the panicle, highly apparent N translocation rate in the leaf and stem-shead with a higher N grain production efficiency and N translocation efficiency, indicating that the accumulation and translocation efficiency of N in the medium-maturing indica hybrid rice cultivars were significantly higher than those in the late-maturing indica hybrid rice cultivars and japonica rice cultivars under the planting mechanization. Fyou498 from medium-maturing indica hybrid rice showed much higher N uptake than the others, and had an advantage of rapid N accumulation at the early growth stage and reasonable N distribution at the late growth stage. 5) Late-maturing indica hybrid rice cultivars demonstrated the biggest enhancement rate in N accumulation from 84 to 91 d, and a steady N accumulation during the whole growth period, and the highest N uptake, but which was not beneficial to yield formation. 6) Cultivar 69you8 has higher N uptakes and yield than the other japonica rice cultivars, and its N accumulation and translocation efficiency was significantly higher under the planting mechanization.【Conclusions】 Different genotypes are significantly different in the characteristics of N accumulation and translocation under machine-transplanting condition. The accumulation and translocation efficiency of N in the medium-maturing indica hybrid rice cultivars is significantly higher than those in the late-maturing indica hybrid rice cultivars and japonica rice cultivars. Fyou498, a medium-maturing indica hybrid rice cultivar, demonstrates genetic advantages in the N accumulation, distribution and yield formation. Late-maturing indica hybrid rice cultivars have potential in high N accumulation, which, however, does not show good to yield formation. N accumulation in the japonica rice cultivars is relatively low. Among the japonica rice cultivars, 69you8 has significantly higher N accumulation and yield under the planting mechanization, showing better suitability for planting mechanization.