• ISSN 1008-505X
  • CN 11-3996/S
蒋文婷, 田立斌, 朱高荻, 唐荣贵, 林永新, 潘灵强, 蔡延江. 不同形态氮添加对毛竹林土壤N2O排放的影响[J]. 植物营养与肥料学报, 2022, 28(5): 857-868. DOI: 10.11674/zwyf.2021492
引用本文: 蒋文婷, 田立斌, 朱高荻, 唐荣贵, 林永新, 潘灵强, 蔡延江. 不同形态氮添加对毛竹林土壤N2O排放的影响[J]. 植物营养与肥料学报, 2022, 28(5): 857-868. DOI: 10.11674/zwyf.2021492
shu
JIANG Wen-ting, TIAN Li-bin, ZHU Gao-di, TANG Rong-gui, LIN Yong-xin, Pan Ling-qiang, CAI Yan-jiang. Effects of different forms of nitrogen addition on N2O emissions from the soil of Moso bamboo (Phyllostachys edulis) forest[J]. Journal of Plant Nutrition and Fertilizers, 2022, 28(5): 857-868. DOI: 10.11674/zwyf.2021492
Citation: JIANG Wen-ting, TIAN Li-bin, ZHU Gao-di, TANG Rong-gui, LIN Yong-xin, Pan Ling-qiang, CAI Yan-jiang. Effects of different forms of nitrogen addition on N2O emissions from the soil of Moso bamboo (Phyllostachys edulis) forest[J]. Journal of Plant Nutrition and Fertilizers, 2022, 28(5): 857-868. DOI: 10.11674/zwyf.2021492
shu

不同形态氮添加对毛竹林土壤N2O排放的影响

Effects of different forms of nitrogen addition on N2O emissions from the soil of Moso bamboo (Phyllostachys edulis) forest

    摘要
  • 摘要:
    目的 氧化亚氮(N2O)排放是亚热带地区氮损失的主要途径，我们研究了不同形态含氮化合物对土壤N2O排放的影响。
    方法 以毛竹(Phyllostachys edulis)林土壤为研究对象进行了室内培养试验。设置土壤中添加KNO3、NH4NO3、NH4Cl、KCl处理，以去离子水作为对照(CK)，在25oC黑暗条件下培养。在培养0.5 h，1、3、5、7、14、28、60天，测定土壤N2O排放速率，铵态氮(NH4+-N)、硝态氮(NO3−-N)、可溶性有机碳(DOC)和水溶性氮(WSN)含量，采用荧光定量PCR技术测定了土壤氨氧化细菌(ammonia-oxidizing bacteria, AOB)、氨氧化古菌(ammonia-oxidizing archaea, AOA)、nirS、nirK、nosZⅠ、nosZⅡ基因丰度。
    结果 培养第60天，氮添加与KCl添加处理均显著增加了土壤DOC含量，NH4NO3、NH4Cl处理显著增加了WSN含量，但显著降低了土壤pH。氮添加及KCl添加处理均增加了土壤AOA、AOB、nirK基因丰度，降低了nosZⅠ、nosZⅡ基因丰度。氮添加处理N2O排放速率均在培养第14天达到峰值，且相较于CK处理均增加了N2O累积排放量，KNO3、NH4NO3、NH4Cl和KCl处理累积排放量的增幅分别为524.3%、771.1%、652.7%、98.6%。N2O排放速率与NO3−、WSN、nirK基因丰度呈显著正相关，而与pH、nosZⅠ、nosZⅡ基因丰度呈显著负相关。
    结论 铵态氮添加能显著促进毛竹林土壤N2O的排放，其效果高于硝态氮，NH4NO3作为混合氮，外源性NH4+-N、NO3−-N同时输入对土壤N2O排放的促进作用比单独添加NH4+-N、NO3−-N更显著，但并未出现叠加效应。

     

    Abstract:
    Objectives Nitrous oxide (N2O) emission is one of the main ways of nitrogen (N) loss in subtropical region, and the effects of different forms of nitrogen compounds on soil N2O emission were investigated in this study.
    Methods A laboratory incubation experiment was conducted using soil from Moso bamboo (Phyllostachys edulis) forest. The N fertilizer treatments were addition of KNO3, NH4NO3, and NH4Cl with equal mole of N input, KCl was added for comparison of K+ and Cl−, and no N addition was set as the control (CK). The soil N2O emission rate, NH4+-N, NO3−-N, soluble organic carbon (DOC) and water-soluble N (WSN) concentrations were measured after 0.5 h, 1 day (d), 3 d, 5 d, 7 d, 14 d, 28 d and 60 d of incubation. On the 14th day of incubation, the gene abundance of ammonia oxidizing bacteria (AOB), ammonia oxidizing archaea (AOA), nirS, nirK, nosZⅠ and nosZⅡ were detected by fluorescence quantitative PCR.
    Results The N fertilizers and KCl addition significantly increased soil DOC concentration, while NH4NO3 and NH4Cl addition significantly increased soil WSN concentration and decreased soil pH after 60 days of incubation. Both N and KCl additions increased soil AOA, AOB, and nirK gene abundance, but decreased nosZⅠ and nosZⅡgene abundance. The N2O emission in all the treatments reached peaks at the 14th day of incubation. Compared with CK, KNO3, NH4NO3, NH4Cl, and KCl increased cumulative N2O emissions by 524.3%, 771.1%, 652.7%, and 98.6%, respectively. The N2O emission rates were significantly and positively correlated with NO3–, WSN, and nirK gene abundance and negatively correlated with pH and nosZⅠ and nosZⅡ gene abundance.
    Conclusions The addition of NH4+-N caused higher soil N2O emission than NO3–-N in Moso bamboo forest. The simultaneous input of NH4+-N and NO3–-N, like NH4NO3, promoted higher emission than their individual inputs, but less than that of the sum of KNO3 and NH4Cl.

     

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