• ISSN 1008-505X
  • CN 11-3996/S
席奇亮, 杨铁钊, 周方, 王冬, 叶红朝, 徐世晓, 杨丙钊. 烟草专用液体肥添加黄腐酸对烤烟西柏三烯二醇代谢水平的影响[J]. 植物营养与肥料学报, 2018, 24(4): 981-991. DOI: 10.11674/zwyf.17339
引用本文: 席奇亮, 杨铁钊, 周方, 王冬, 叶红朝, 徐世晓, 杨丙钊. 烟草专用液体肥添加黄腐酸对烤烟西柏三烯二醇代谢水平的影响[J]. 植物营养与肥料学报, 2018, 24(4): 981-991. DOI: 10.11674/zwyf.17339
shu
XI Qi-liang, YANG Tie-zhao, ZHOU Fang, WANG Dong, YE Hong-chao, XU Shi-xiao, YANG Bing-zhao. Effect of the liquid fertilizer containing fulvic acid on metabolism of cembratrien-diols in flue-cured tobacco[J]. Journal of Plant Nutrition and Fertilizers, 2018, 24(4): 981-991. DOI: 10.11674/zwyf.17339
Citation: XI Qi-liang, YANG Tie-zhao, ZHOU Fang, WANG Dong, YE Hong-chao, XU Shi-xiao, YANG Bing-zhao. Effect of the liquid fertilizer containing fulvic acid on metabolism of cembratrien-diols in flue-cured tobacco[J]. Journal of Plant Nutrition and Fertilizers, 2018, 24(4): 981-991. DOI: 10.11674/zwyf.17339
shu

烟草专用液体肥添加黄腐酸对烤烟西柏三烯二醇代谢水平的影响

Effect of the liquid fertilizer containing fulvic acid on metabolism of cembratrien-diols in flue-cured tobacco

    摘要
  • 摘要:
    目的 西柏三烯二醇是烤烟香味成分的重要前提物质。本研究探究了黄腐酸对烟草西柏三烯二醇代谢的影响，为提高烤烟品质提供有效方法。
    方法 以烤烟品种‘豫烟6号’为试材，连续进行了2年的田间试验和1年的盆栽试验。设常规栽培 (FP)、普通烟草专用液体肥料 (DF1) 和含黄腐酸烟草专用液体肥料 (DF2) 3个处理，两种液体肥采用水肥一体化方法施用。观察了不同处理的腺毛形态和密度，测定了不同处理的叶面腺毛分泌物，并利用荧光定量PCR技术比较了不同处理与西柏三烯二醇代谢相关的CYC-1、CYP71D16、DXR、DXS等基因的表达量，对比了不同处理初烤烟叶的茄酮含量并进行了感官质量评价。
    结果 1) DF2处理的腺毛密度较高且具有较强分泌能力的长柄腺毛较多，叶面腺毛分泌物中西柏三烯二醇的含量也表现为DF2 (65.43 μg/cm2) > FP (46.84 μg/cm2) > DF1 (39.8 μg/cm2)；2)DF2处理能显著提高烟叶CYC-1、CYP71D16、DXR、DXS基因的表达量，4个基因的表达量分别是FP处理的12.45倍、3.54倍、3.22倍和3.44倍，是DF1处理的4.11倍、5.71倍、2.44倍和2.28倍；3) DF2处理调制后的初烤烟叶茄酮含量明显高于其他处理，可达到112.27 μg/g，分别是FP和DF1处理的2.93倍和1.99倍，且连续2年感官质量评价结果均优于其他处理。
    结论 肥料中的黄腐酸能促进烤烟西柏三烯二醇代谢相关基因的上调表达，改良烤烟叶面分泌物，提高烟叶茄酮含量，改善抽吸品质。

     

    Abstract:
    Objectives This experiment was conducted to investigate the effect of the new liquid fertilizer containing fulvic acid for tobacco on metabolism of cembratrien-diols in flue-cured tobacco.
    Methods Flue-cured tobacco cultivar ‘Yuyan 6’ was used as material. The treatments included conventional practice (FP), ordinary liquid fertilizer for tobacco (DF1) and liquid fertilizer containing fulvic acid for tobacco (DF2), which were all applied with drip irrigation. The morphology and density of glandular hairs were observed, the glandular trichome exudates were measured, the relative expressions of CYC-1, CYP71D16, DXR and DXS were determined using RT-qPCR, and the content of solanone and sensory quality were evaluated.
    Results 1) The trichome density in DF2 treatment was higher and the number of long handle trichomes was more than the other two treatments. The content of cembratrien-diols in glandular trichome exudates was in order of DF2 (65.43 μg/cm2) > FP (46.84 μg/cm2) > DF1(39.8 μg/cm2); 2) The relative expression of CYC-1, CYP71D16, DXR and DXS in DF2 treatment were significantly higher than in DF1 treatment, which were 12.45 times, 3.54 times, 3.22 times and 3.44 times than FP treatment and 4.11 times, 5.71 times, 2.44 times and 2.28 times of those in DF1, respectively; 3) The content of solanone in modulated flue-cured tobacco leaves in DF2 treatment was higher than in the other treatments and reached 112.27 µg/g, which is 2.93 times and 1.99 times of that in FP and DF1, respectively. The sensory quality of tobacco in DF2 treatment was better than in the other treatments in 2015 to 2016.
    Conclusions The fulvic acid contained in the liquid fertilizer stimulates the expression levels of genes related cembratrien-diols synthesis, meliorated foliar secretion of flue cured tobacco and improved the content of solanone and the sensory quality evaluation.

     

    • HTML全文
    • 参考文献(40)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回