Abstract: Based on long term experiment of fertilization, analysis soil sulfur fertility factor developing with time and supplied S ability of red paddy soil with different fertilization constitution. The result showed that fertilization constitution obviously affected developing with time of total S, organic S, inorganic S and inorganic S plus fraction organic S (40 ℃ KCl extracted) of soil, and sulfur mineralization potential, mineralization rate constant and half time for S mineralization. Applied straw and organic manure with chemical fertilizer helped to soil S accumulate in soil and increase supplied S ability. Under greenhouse conditions, arranged order of treatments was straw return to fieldorganic manure plus inorganic fertilizertotal inorganic fertilizercontrol, according S uptake from different soil treated by different fertilization constitution. It was as same as orders of soil total S, inorganic S plus fraction organic S and sulfur mineralization potential.