Abstract: Some phosphate solubilizing microorganisms were grown in liquid medium cultures with different nitrogen materials (NH4+, NO3-, urea), carbon materials (glucose, sucrose, molasses, starch) and C/N ratios (34∶1, 20∶1, 5∶1). Of the eight isolates tested,two isolates of Aspergillus sp. 2TCiF2 and 4TCiF6 had much higher capacity to dissolve rock phosphate when supplying with NO3- than with either NH4+ or urea. However, Arthrobacter sp.1TCRi7 and 1TCRi14 decreased their activities of dissolving rock phosphate at NO3- medium. Other isolates of Penicillium sp.1TCRiF5 and 2TCRiF4, Enterobacter sp.1TCRi15 and Erwinia sp.4TCRi22 showed strong ability to solubilize rock phosphate only at NH4+ medium. Little effect was found on phosphate solubilization when adding a small amount of available phosphate in the culture media. Carbon sources had strong influence on rock phosphate solubilization. The fungus of Penicillium sp.2TCiF2 showed the highest capacity to dissolve the rock when supplying sucrose. However, a bacterium of Arthrobacter sp.1TCRi7 had this ability only in the glucose medium. The isolates of Aspergillus sp. and Erwinia sp. had higher capacity of phosphate solubilization in larger C/N ratios of the media, but Penicillium sp. and Enterobacter sp. showed the highest activity in the lowest C/N ratio. The effect of C/N ratios was little in phosphate solubilization of rthrobacter sp. It is supposed that complexion of organic acids with metals may be the main reason for these isolates to solubilize the phosphate. In particular, other chelant substances may be much more important for Enterobacter sp. and Erwinia sp. to release P from the rock phosphate. Metabolisms and especially secretion of the nature of the organic acids by these microorganisms were greatly affected by nitrogen sources, carbon sources and C/N ratios.