Abstract: In order to discuss the effects of bio-organic manure on ginger（Zingiber officinale Rosc.）yield, the leaf photosynthesis and chlorophyll fluorescence in different fertilizer treatments were studied in the experiment using the cultivar ‘Laiwu da jian’. The results indicated that bio-organic manure could increase leaf pigment content and photosynthetic rate, and promote the growth of ginger. Moreover, the effects of bio-organic manure improved with ginger growth. For instance, at the vigorous growth stage (22 Sep.), the leaf chlorophyll content of ginger with bio-organic manure application was 5.5% and 13.1% higher than that of common manure and chemical fertilizer, respectively, and the photosynthetic rate was 8.2% and 17.8% higher, respectively. Though the leaf photochemical efficiency of ginger decreased under the condition of high temperature and strong light at noon, the leaf intrinsic photochemical efficiency (Fv/Fm), quantum yield of PSⅡ (φPSⅡ) and photochemical quenching (qP) of ginger applied with bio-organic manure were higher than that of common manure and chemical fertilizer, and non-photochemical quenching (NPQ) was lower. This indicated that bio-organic manure could enhance light utilization efficiency of ginger leaves. Yield of ginger with bio-organic manure application was 9% and 24% higher than that of common manure and chemical fertilizer, respectively.