Abstract: The field experiments were conducted to investigate the effects of different nitrogen（N） fertilizer rates and the ratio of basal N and topdressing N on winter wheat growth, grain yield and the change of soil N content in Hengshui and Xinji, Hebei province, respectively. The results show that indigenous N supply could meet the demand of N for winter wheat normal growth before the revival stage, and the applied N fertilizer do not significantly increase wheat grain yield in Hengshui site, while compared with the treatments of applying basal N and topdressing N, the applications of topdressing N only at shooting and booting stages at the ratio of 2:1 increase N use efficiency significantly. In Xinji site, 60 kg N /ha could meet the demand of N for wheat growth before the shooting stage, all N are applied as topdressing at the shooting stage do not eliminate the effect of N deficient at the early growth stage on wheat growth and high grain yield, and the application of 240 kg N/ha at the ratio of 1:3 (basal N and topdressing N ) at the shooting stage could achieve higher grain yield and N use efficiency compared with other treatments. The apparent N loss during winter wheat growth season mainly comes from the basal N, while high topdressing N rates at mid-later stages also increase soil N residual after the harvest. Because of the variations of soil fertility and texture, the effects of the same N management method on winter wheat growth, yield and N use efficiency in two sites are different, so for higher crop yield and higher N use efficiency, the synchronizing regulation of N fertilizer should combine crop nutrient demand, nutrient supply from soil and regional variation characters of soil fertility and texture.