Abstract: 【Objectives】Root interaction is closely related to nutrients uptakes of crops in intercropping systems. The study was to find out phosphorus absorption and accumulation of maize and soybean in the maize and soybean intercropping system. 【Methods】A pot experiment of maize and soybean intercropping was carried out with root separation treatments, no barrier(NB), mesh barrier(MB) and polythene film barrier(PB). The absorption of phosphorus in different growth periods of the maize(seeding stage, large bell stage, booting stage and maturity stage)and soybean(seeding stage, branching stage, filling stage and maturity stage) were analysed.【Results】 In comparison with PB, the phosphorus content in stem of maize at the booting stage in MB was increased by 16.8%(P＜0.05)and that in leaf at the seeding stage and large bell stage increased by 61.6%,14.2% respectively(P＜0.05); The phosphorus contents in stem of maize at the large bell stage and booting stage in NB were increased by 25.4% and 55.0% respectively(P＜0.05), and those in leaves at the large bell stage and maturity stage were increased by 21.3% and 22.2% respectively(P＜0.05). In comparison with PB, the phosphorus contents in stem of soybean at the branching and maturity stage in NB were increased by 21.3% and 24.7% respectively(P＜0.05)and those in leaves at the branching stage was increased by 11.4%(P＜0.05). The leaf phosphorus content of soybean at the branching stage in MB was increased by 4.8%(P＜0.05). In comparison with PB, The stem phosphorus accumulation amounts of maize with NB at the large bell stage and booting stage increased by 24.6% and 32.3% respectively(P＜0.05), and the stem phosphorus accumulation amount of maize with MB at the large bell stage was increased by 50.6%(P＜0.05)and the leaves phosphorus accumulation amount of maize at the seeding stage is increased by 33.6%(P＜0.05). In comparison with PB, the stems phosphorus accumulation amounts of soybean with NB at the branching stage and filling stage are increased by 36.3% and 51.8% respectively(P＜0.05)and the leaf phosphorus accumulation amounts of soybean at the branching stage and maturity stage are increased by 27.3% and 110.6% respectively(P＜0.05), and the stem phosphorus accumulation amount of soybean with MB at the filling stage is increased by 35.7%(P＜0.05).【Conclusions】 In intercropping system, the stem and leaf phosphorus accumulation amounts of maize are significantly higher than those in seperated root enviroment, showing an obvious phosphorans nutrition and yield advantage.