• ISSN 1008-505X
  • CN 11-3996/S
李韬, 孙发宇, 龚盼, 王安, 袁林喜, 尹雪斌. 施纳米硒对小麦籽粒硒含量及其品质性状的影响[J]. 植物营养与肥料学报, 2017, 23(2): 427-433. DOI: 10.11674/zwyf.16217
引用本文: 李韬, 孙发宇, 龚盼, 王安, 袁林喜, 尹雪斌. 施纳米硒对小麦籽粒硒含量及其品质性状的影响[J]. 植物营养与肥料学报, 2017, 23(2): 427-433. DOI: 10.11674/zwyf.16217
LI Tao, SUN Fa-yu, GONG Pan, WANG An, YUAN Lin-xi, YIN Xue-bin. Effects of nano-selenium fertilization on selenium concentration of wheat grains and quality-related traits[J]. Journal of Plant Nutrition and Fertilizers, 2017, 23(2): 427-433. DOI: 10.11674/zwyf.16217
Citation: LI Tao, SUN Fa-yu, GONG Pan, WANG An, YUAN Lin-xi, YIN Xue-bin. Effects of nano-selenium fertilization on selenium concentration of wheat grains and quality-related traits[J]. Journal of Plant Nutrition and Fertilizers, 2017, 23(2): 427-433. DOI: 10.11674/zwyf.16217

施纳米硒对小麦籽粒硒含量及其品质性状的影响

Effects of nano-selenium fertilization on selenium concentration of wheat grains and quality-related traits

  • 摘要:
    目的研究基施纳米硒肥对不同小麦品种籽粒百粒重、硒含量、硒形态,其他矿质元素以及面粉糊化特性的影响,为利用纳米硒肥进行小麦硒生物强化提供参考。
    方法选择 110 份小麦品种 (系),在扬州大学农学院网室种植,采用的施肥方式为基施纳米硒肥,设每千克土施 Se 0 (CK)、100 (Se100)、150 (Se150) mg 2 次重复,随机区组设计。小麦成熟期收获籽粒,测定百粒重。利用离子发射光谱 – 原子吸收仪测定了籽粒 Se、Ca、Mg、Cu、Fe、Mn、Zn 及 S 含量。选择高硒处理中硒含量前 10 名的品种,利用 LC-UV-AFS 测定了籽粒中不同形态硒的含量。随机挑选 59 个品种并利用 RVA 仪 (快速粘度分析仪) 测定其面粉糊化特性的特征值。利用 MATLAB 和 SPSS 软件进行数据分析。
    结果基施硒 0、100、150 mg/kg 土,小麦籽粒的百粒重均值分别为 3.78、4.11 和 3.70 g,籽粒中总硒含量分别为 2.00、12.46 和 17.35 mg/kg。籽粒中的硒主要以有机态形式存在,以硒蛋氨酸含量最多,其次是硒甲基化半胱氨酸和少量的硒半胱氨酸,无机态硒仅微量存在于极少数品种中。基施硒肥对测定的矿质元素的吸收积累既有协同也有拮抗作用,对小麦面粉的糊化特性没有显著影响。糊化特性取决于基因型,不同品种间存在极显著差异。
    结论硒肥可以提高小麦籽粒中的硒含量,但增幅因品种而异;硒强化后籽粒中硒主要以有机态形式存在;低浓度硒处理可以提高小麦籽粒的百粒重,且不会影响小麦的面粉糊化特性。

     

    Abstract:
    ObjectivesThis article reported the influences of selenium application on the total and different forms of selenium concentration in wheat grains, which would clarify the potential of biofortification of Se in wheat through fertilization.
    MethodsPot experiment was conducted and 110 wheat cultivars were collected as materials. The nano-Se was applied. Three levels of Se 0 (CK), 100 (Se100) and 150 mg/kg soil (Se 150) were designed and applied uniformly into soil before wheat planting. The 100-kernel weight was investigated. The concentrations of Se, Ca, Mg, Cu, Fe, Mn, Zn and S were measured using ICP. Using LC-UV-AFS method, the forms of Se were identified and quantified in the cultivars with total Se content in top ten, which were under treatment of Se150. Fifty-nine cultivars were randomly selected for paste viscosity evaluation using Rapid ViscoAnalyser. Data were analyzed using softwares Matlab2014 and SPSS16.
    ResultsThe mean 100-kernel weight for CK, Se100 and Se150 was 3.78, 4.11, and 3.70 g, respectively, and the total Se concentration was averaged 2.00, 12.46 and 17.35 mg/kg, respectively. Se was mainly existed in organic forms, of which selenomethionin (SeMet) was the predominant form, and then were methylated selenocysteine (MeSeCys) and selenocysteine (SeCys). Trace amounts of inorganic forms of Se4+ and Se6+ were also identified in some cultivars. Application of nano-Se fertilizer had either synergistically or antagonistically effects on the accumulation of the tested nutrients, but did not on the flour paste viscosity properties, which were mainly determined by genotypes of the cultivars.
    ConclusionsFertilization of nano-Se markedly increased the concentration of Se in wheat grains. Se in grains predominantly presented in organic forms of selenomethionin. Application of Se 100 mg/kg soil is effective in increment of 100-kernel weight, and application of Se 150 mg/kg soil will reduce it. Se fertilization had not significant impact on wheat flour paste viscosity properties.

     

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