• ISSN 1008-505X
  • CN 11-3996/S
王岩, 韩苗, 熊子怡, 郭涛. 丛枝菌根真菌对玉米锌、磷拮抗作用的影响[J]. 植物营养与肥料学报, 2018, 24(1): 279-284. DOI: 10.11674/zwyf.16438
引用本文: 王岩, 韩苗, 熊子怡, 郭涛. 丛枝菌根真菌对玉米锌、磷拮抗作用的影响[J]. 植物营养与肥料学报, 2018, 24(1): 279-284. DOI: 10.11674/zwyf.16438
WANG Yan, HAN Miao, XIONG Zi-yi, GUO Tao. Influence of arbuscular mycorrhizal fungus on the antagonistic action of zinc and phosphorus in maize[J]. Journal of Plant Nutrition and Fertilizers, 2018, 24(1): 279-284. DOI: 10.11674/zwyf.16438
Citation: WANG Yan, HAN Miao, XIONG Zi-yi, GUO Tao. Influence of arbuscular mycorrhizal fungus on the antagonistic action of zinc and phosphorus in maize[J]. Journal of Plant Nutrition and Fertilizers, 2018, 24(1): 279-284. DOI: 10.11674/zwyf.16438

丛枝菌根真菌对玉米锌、磷拮抗作用的影响

Influence of arbuscular mycorrhizal fungus on the antagonistic action of zinc and phosphorus in maize

  • 摘要:
    目的 利用丛枝菌根 (arbuscular mycorrhizal fungi,AM) 真菌与作物互利共生的关系来提高作物对锌的吸收是缓解锌、磷拮抗作用的途径之一,本试验在不同锌、磷浓度条件下,研究了接种AM真菌对玉米侵染和锌、磷吸收的影响,以期为揭示AM真菌影响锌、磷拮抗作用的机理提供理论依据。
    方法 采用盆栽试验,设置三个施磷水平 (0、200 、400 mg/kg),两个施锌水平 (0、5 mg/kg),2个接菌水平接菌 (+AM)和不接菌 (–AM),共12个处理,每个处理4次重复。利用生物镝灯补充光照,在人工光照植物培养室内植株生长50天后,地上部与根部分别收获,测定其生物量、锌磷的含量和吸收量。
    结果 施磷和接种AM真菌都显著提高了玉米植株生物量,不施锌条件下,施磷从0 mg/kg增加到400 mg/kg,玉米植株地下部和地上部生物量分别提高6.67倍、9.30倍。接种处理对玉米植株生物量的影响也有相同的趋势。在锌水平为5 mg/kg、磷水平为200 mg/kg的条件下,接种AM真菌玉米植株地下部磷的吸收量和含量分别增加了110%、55%;在同一锌、磷供给条件下,接种AM真菌显著提高了玉米对锌的吸收量,地下部和地上部分别是未接种处理的1.71倍和1.68倍。随着施磷水平的不断提高,玉米植株的锌含量会逐渐下降。不施锌条件下,施磷从0 mg/kg增加到200 mg/kg,玉米植株地上部锌含量降低36%,与之相反,接种AM真菌后地上部锌含量增加35%。但在高磷条件 (400 mg/kg) 下,接种AM真菌对玉米植株锌磷含量和吸收量影响均不显著。
    结论 在本试验条件下,施磷抑制玉米对锌的吸收,接种AM真菌可提高玉米锌磷的含量和吸收量,有效缓解玉米锌磷拮抗作用,改善玉米的锌营养状况。

     

    Abstract:
    Objectives Improving Zn utilization efficiency for crops using arbuscular mycorrhizal symbiosis is one of the effective ways for alleviating the antagonism of zinc and phosphorus. It is well established that simultaneous application of Zn and phosphorus (P) fertilizers has antagonistic effects on plant Zn uptake. Arbuscular mycorrhizas (AM) can improve plant Zn and P uptake. We conducted screenhouse experiment to test the effect of different levels of P in conjunction with mycorrhizal colonization on plant nutrition and biomass.
    Methods A pot experiment was conducted with maize as the host plants in soil and three levels of phosphorus (0, 200 and 400 mg/kg), two levels of zinc (0 and 5 mg/kg) application, which included inoculation and inoculation. A total of 12 treatments were designed with 4 replicates. Plant biomass, Zn and P contents, mycorrhizal colonization after fifty days were measured.
    Results Colonization by AMF and the application of phosphorus increased maize plant biomass significantly. Under the condition of no zinc application, the biomass of shoots and roots of maize were respectively increased by 6.67 times and 9.30 times when the application of phosphorus increased from 0 to 400 mg/kg, no matter inoculating AMF or not. When supplying zinc 5 mg/kg and P 200 mg/kg, the mycorrhizal inoculation increased the uptake and concentration of phosphorous in maize roots by 110% and 55% respectively, increased zinc uptake in roots and shoots by 71% and 68% respectively. Without Zn application, the zinc contents of maize plants were gradually decreased with the increasing of P application levels. When P application level was increased from 0 mg/kg to 200 mg/kg, the Zn content in maize shoot was decreased by 36%, while that was increased by 35% after the inoculation of AM fungi. When the phosphorus application level was as high as 400 mg/kg, inoculation of AM fungi was unable to influence the contents of Zn and P in maize plants significantly.
    Conclusions Mycorrhizal inoculation could relief the antagonistic effect of phosphorus on zinc uptake of maize to some extent.

     

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