• ISSN 1008-505X
  • CN 11-3996/S
孙逸萌, 安华燕, 韩效钊, 孙敏. C2-C4有机酸和醇作为潜在种子引发剂:基于灰色关联分析的综合评估[J]. 植物营养与肥料学报, 2018, 24(3): 790-804. DOI: 10.11674/zwyf.17351
引用本文: 孙逸萌, 安华燕, 韩效钊, 孙敏. C2-C4有机酸和醇作为潜在种子引发剂:基于灰色关联分析的综合评估[J]. 植物营养与肥料学报, 2018, 24(3): 790-804. DOI: 10.11674/zwyf.17351
SUN Yi-meng, AN Hua-yan, HAN Xiao-zhao, SUN Min. C2-C4 organic acids and alcohols as potential seed priming chemicals: A comprehensive evaluation based on grey relational analysis[J]. Journal of Plant Nutrition and Fertilizers, 2018, 24(3): 790-804. DOI: 10.11674/zwyf.17351
Citation: SUN Yi-meng, AN Hua-yan, HAN Xiao-zhao, SUN Min. C2-C4 organic acids and alcohols as potential seed priming chemicals: A comprehensive evaluation based on grey relational analysis[J]. Journal of Plant Nutrition and Fertilizers, 2018, 24(3): 790-804. DOI: 10.11674/zwyf.17351

C2-C4有机酸和醇作为潜在种子引发剂:基于灰色关联分析的综合评估

C2-C4 organic acids and alcohols as potential seed priming chemicals: A comprehensive evaluation based on grey relational analysis

  • 摘要:
    目的 种子作为植物生长发育的起点,承载了全部的遗传因素以及作物发芽所需要的营养物质,其质量的好坏很大程度上影响着植物的后续发展以及最终的产量。化学浸种处理是促进幼苗快速,均匀田间出苗的有效途径。寻求环境友好和经济可行的化学品应用于种子引发,可为促进作物生长提供一条经济有效的途径。
    方法 以低成本的C2-C4有机酸和醇作为氨基酸的替代化学浸种剂,进行了玉米、小麦和绿豆种子浸种试验。在试验研究中,甘氨酸、乙酸盐、丙酸盐、丁酸盐的各供试浓度同为10、20、30、40、50 mg/L,乙醇、丙醇和丁醇的各供试浓度同为3、5、8、10、13 mg/L。在25℃下浸泡24 h,然后置于25℃和80%湿度的环境下待发芽,5 d后计算发芽率,2周后测量枝条长度、根长度及幼苗生物量。使用灰色关联分析来整合发芽率、枝条长度、根长度和幼苗生物量对作物后续生长的影响,将发芽率的权重设为0.4,枝条长度、根长度及幼苗生物量的权重均设为0.2。依据归一化数据计算出的灰色关联系数,对引发化学物进行了全面评估。
    结果 将数据带入公式进行计算,20 mg/L乙酸盐获得0.797的最高引发等级,作为诱导玉米种子的最佳化学试剂,而甘氨酸的最佳浓度为40 mg/L,引发等级为0.701;40 mg/L丙酸盐在绿豌豆种子上获得0.730最高引发等级,在小麦种子上获得0.799的最高引发等级,高于最佳浓度为30 mg/L的甘氨酸处理中获得的引发等级0.699。
    结论 这项工作证明了低分子有机酸和醇是合适的生长调节物质。以40 mg/L 乙酸盐在玉米、40 mg/L丙酸盐在小麦和绿豆上的效果最佳。

     

    Abstract:
    Objectives Chemical seed priming treatment is an effective approach to facilitate the rapid and uniform field emergence of seedlings. Seeking for environmentally friendly and economically feasible chemicals for seed priming application affords an alternative way for higher crop yields.
    Methods Six C2-C4 organic acids and alcohols including acetate, propionate, butyrate, ethanol, propanol and butanol were used to treat the seeds of corn, green pea and wheat. The used treatment concentrations for acetate, propionate, butyrate were 10, 20, 30, 40 and 50 mg/L, and those for ethanol, propanol and butanol were 3, 5, 8, 10 and 13 mg/L. The tested seeds were all soaked in the solutions for 24 h at 25℃, then placed at 25℃ under moisture of 80% for germination. The seed germination rate were investigated at the fivth day, and the lenghth of sprouts and roots, and the biomass of seedlings were measured after two weeks of the germination. and development are evaluated. Grey relational analysis is employed to integrate the germination percentage, shoot length, root length and seedling weight for a comprehensive assessment of the priming chemicals.
    Results The 20 mg/L acetate was proved optimal for the seeds of corn, its priming grade was 0.797, higher than that of the 40 mg/L of glycine (0.701); The propionate of 40 mg/L obtained the same priming grade of 0.730 with glycine on green pea seeds, which was the highest priming grade that glycine could produced on green pea seeds, and the propionate of 40 mg/L also produced the highest priming grade of 0.799 on wheat, which was better than the best grade of 0.699 obtained by the 30 mg/L glycine.
    Conclusions This work demonstrates the low molecular organic acids and alcohols are available to be used as seed priming chemicals. Among the tested chemicals, 40 mg/L of acetate and 40 mg/L of propionate performs best on corn seeds and wheat and green pea seeds respectively.

     

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