• ISSN 1008-505X
  • CN 11-3996/S
张倩, 陈为峰, 董元杰. 外源硝普钠与EDTA强化黑麦草耐镉性及镉积累[J]. 植物营养与肥料学报, 2019, 25(9): 1560-1568. DOI: 10.11674/zwyf.18380
引用本文: 张倩, 陈为峰, 董元杰. 外源硝普钠与EDTA强化黑麦草耐镉性及镉积累[J]. 植物营养与肥料学报, 2019, 25(9): 1560-1568. DOI: 10.11674/zwyf.18380
ZHANG Qian, CHEN Wei-feng, DONG Yuan-jie. Adding sodium nitroprusside and EDTA to strengthen cadmium tolerance and cadmium accumulation of ryegrass[J]. Journal of Plant Nutrition and Fertilizers, 2019, 25(9): 1560-1568. DOI: 10.11674/zwyf.18380
Citation: ZHANG Qian, CHEN Wei-feng, DONG Yuan-jie. Adding sodium nitroprusside and EDTA to strengthen cadmium tolerance and cadmium accumulation of ryegrass[J]. Journal of Plant Nutrition and Fertilizers, 2019, 25(9): 1560-1568. DOI: 10.11674/zwyf.18380

外源硝普钠与EDTA强化黑麦草耐镉性及镉积累

Adding sodium nitroprusside and EDTA to strengthen cadmium tolerance and cadmium accumulation of ryegrass

  • 摘要:
    目的 黑麦草常用作土壤镉污染修复植物,本文研究外源添加硝普钠 (一氧化氮供体,SNP) 与乙二胺四乙酸 (EDTA) 提高黑麦草耐镉性和镉积累量的效应,以期明确外源SNP和EDTA强化黑麦草修复土壤Cd污染的效应与机制。
    方法 以黑麦草品种‘绅士’为试验材料,SNP和EDTA作为调控物质进行了液培试验。试验以Hoagland营养液为基础,设定正常营养液 (CK) 和含CdCl2 100 μmol/L的胁迫营养液 (Cd),并在两种营养液中分别加入100 μmol/L SNP、0.5 mmol/L EDTA、0.25 mmol/L EDTA + 50 μmol/L SNP,共8个处理。幼苗处理18天后,调查了黑麦草生长指标、镉含量、根系活力、光合色素含量、\rmO_\small 2^\overline \,\cdot\, 产生速率、H2O2含量、丙二醛含量、电解质渗出率及抗氧化酶活性。
    结果 非镉胁迫下,外源添加EDTA和SNP均显著降低黑麦草株高、根长及根系活力,同时显著提高了黑麦草地上、地下部\rmO_\small 2^\overline \,\cdot\, 产生速率、H2O2和MDA含量。100 μmol/L Cd严重抑制了黑麦草的生长。添加SNP与EDTA可缓解镉胁迫对黑麦草的伤害,二者复合处理效果明显。与Cd处理相比,Cd + EDTA + SNP处理的株高、根长分别增加了68.8%和59.6%,鲜重和干重分别增加了62.6%和60.0%;地上与地下部SOD活性分别提高了32.5%、67.6%,POD活性分别提高了49.8%、67.6%,APX酶活性分别提高了134%、102%,MDA含量分别降低了30.4%、21.8%,\rmO_\small 2^\overline \,\cdot\, 产生速率分别降低了29.0%、26.1%;同时,地上部与地下部镉含量分别显著提高了89.7%和30.2%。
    结论 非镉胁迫下添加100 μmol/L SNP、0.5 mmol/L EDTA、0.25 mmol/L EDTA + 50 μmol/L SNP均对黑麦草生长有一定抑制作用。镉胁迫下,外源添加SNP与EDTA能够在促进黑麦草镉吸收、提高其地上部镉积累量的同时增强其耐镉性,有效缓解镉胁迫对黑麦草生长的抑制作用。本研究中,在提高黑麦草抗Cd胁迫能力和积累Cd量方面以同时添加0.25 mmol/L EDTA和50 μmol/L SNP的效果最佳。

     

    Abstract:
    Objectives Ryegrass is an effective bioremediation plant of Cadmium (Cd) contamination soil. The effects of exogenous sodium nitroprusside (SNP) and EDTA were tested to increase the Cd tolerance and accumulation of ryegrass. In order to clarify the effect and mechanism of exogenous SNP and EDTA in strengthening ryegrass remediation of soil Cd contamination.
    Methods Hydroponic culture method was used with the Hoagland nutrition solution as base, the effects of the application of exogenous SNP and EDTA on growth, physiological characteristics and cadmium accumulation of ryegrass under the stress of 100 μmol/L Cd were studied with ryegrass (Lolium perenne L.) as the experimental material, sodium nitroprusside (SNP) as the donor of nitric oxide (NO) and EDTA as the regulatory substance. There were eight treatments, CK, Cd, EDTA, SNP, Cd + EDTA, Cd + SNP, EDTA + SNP and Cd + EDTA + SNP. The effects of SNP and EDTA on cadmium content, growth index, root activity, photosynthetic pigment content, \rmO_\small 2^\overline \,\cdot\, generation rate, H2O2 content, malondialdehyde content, electrolyte leakage and antioxidant enzyme activity of ryegrass under the cadmium stress were studied.
    Results Under non-stress conditions, exogenous addition of EDTA and SNP significantly reduced the plant height, root length and root activity of ryegrass, and significantly increased the \rmO_\small 2^\overline \,\cdot\, generation rate, H2O2 and MDA content in the above-ground and underground parts of ryegrass. The Cd stress significantly inhibited the growth of ryegrass. The addition of SNP and EDTA could alleviate the damage and their combined treatment had better effect. Compared with the Cd control, the shoot height and root length of the Cd + EDTA + SNP treatment were increased by 68.8% and 59.6%, and the fresh and dry weight were increased by 62.6% and 60.0% respectively. The SOD activities of shoots and roots were increased by 32.5% and 67.6%, the POD activities were increased by 49.8% and 67.6%, the APX enzyme activities were increased by 134% and 102%, the MDA contents were decreased by 30.4% and 21.8%, and the \rmO_\small 2^\overline \,\cdot\, generation rates were decreased by 29.0% and 26.1%, respectively. At the same time, the contents of cadmium in shoots and roots were increased by 89.7% and 30.2%, respectively, and the difference was significant.
    Conclusions The addition of 100 μmol/L SNP, 0.5 mmol/L EDTA, 0.25 mmol/L EDTA and 50 μmol/L SNP has inhibitory effects on the growth of ryegrass under non-stress. The addition of both SNP and EDTA can promote the uptake and accumulation of Cd in the aboveground parts of ryegrass, enhance its tolerance to Cd and effectively alleviate the inhibiting effect of Cd stress on ryegrass growth. The simultaneous application of EDTA and sodium nitroprusside is proved of more efficient than the individual does, as a result, is recommended in the practical use.

     

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