• ISSN 1008-505X
  • CN 11-3996/S
郭向阳, 陈建军, 卫晓轶, 吴迅, 祝云芳, 王安贵, 刘鹏飞, 汤继华, 陈泽辉, 罗红兵. 氮胁迫与非胁迫条件下玉米叶形相关性状的QTL分析[J]. 植物营养与肥料学报, 2019, 25(11): 1929-1938. DOI: 10.11674/zwyf.18507
引用本文: 郭向阳, 陈建军, 卫晓轶, 吴迅, 祝云芳, 王安贵, 刘鹏飞, 汤继华, 陈泽辉, 罗红兵. 氮胁迫与非胁迫条件下玉米叶形相关性状的QTL分析[J]. 植物营养与肥料学报, 2019, 25(11): 1929-1938. DOI: 10.11674/zwyf.18507
GUO Xiang-yang, CHEN Jian-jun, WEI Xiao-yi, WU Xun, ZHU Yun-fang, WANG An-gui, LIU Peng-fei, TANG Ji-hua, CHEN Ze-hui, LUO Hong-bing. QTL mapping of leaf related traits of maize with or without nitrogen stress[J]. Journal of Plant Nutrition and Fertilizers, 2019, 25(11): 1929-1938. DOI: 10.11674/zwyf.18507
Citation: GUO Xiang-yang, CHEN Jian-jun, WEI Xiao-yi, WU Xun, ZHU Yun-fang, WANG An-gui, LIU Peng-fei, TANG Ji-hua, CHEN Ze-hui, LUO Hong-bing. QTL mapping of leaf related traits of maize with or without nitrogen stress[J]. Journal of Plant Nutrition and Fertilizers, 2019, 25(11): 1929-1938. DOI: 10.11674/zwyf.18507

氮胁迫与非胁迫条件下玉米叶形相关性状的QTL分析

QTL mapping of leaf related traits of maize with or without nitrogen stress

  • 摘要:
    目的 叶片是植物光合作用的重要器官,也是蒸腾作用和抗逆的主要器官。在施氮 (N+) 与不施氮 (N–) 条件下鉴定玉米叶片相关性状的QTL,为高光效玉米新品种选育提供重要的理论依据。
    方法 利用玉米骨干系综3为供体,许178为受体,通过杂交、回交和分子标记辅助选择的方法,构建了一套以许178为背景的综3单片段代换系 (SSSLs) 群体,其中包含160个单片段代换系。以这套SSSLs以及许178为材料,在施氮和不施氮条件下,通过一年三点 (贵州贵阳、德江和云南罗平) 的表型评价,利用复合区间作图法对叶面积、叶绿素含量和穗下绿叶数3个叶片相关性状进行QTL定位。
    结果 在基因组范围内,两种氮处理条件下共检测到42个主要叶片相关性状QTLs,分布于10条染色体上。N+条件下,在3个地点共检测出8个叶面积的QTLs,5个穗下绿叶数的QTLs,8个叶绿素QTLs。其中,qLAI1b在3个环境中同时被检测到;qLAI1b在德江、贵阳和罗平点对叶面积的贡献率分别为14.41%、14.47%和16.38%,来自于综3的等位基因起增效作用。同时,穗下绿叶数QTL (qLN7aqLN7b) 在3个环境中均被检测到。在氮胁迫 (N–) 条件下,3个环境中共检测出9个叶面积QTLs,7个穗下绿叶数QTLs,8个叶绿素QTLs;其中,位于bin3.08的叶面积QTL qLAI3b,片段大小为120.48 cM,在德江、贵阳和罗平的贡献率分别为20.4%、12.8%和13.2%,来自于许178的等位基因起增效作用;玉米穗下绿叶数QTL,位于bin9.01区的穗下绿叶数QTL qLN9 (umc1957~umc1867~umc2078),片段大小为62.7 cM。位于bin4.08的叶绿素含量QTL qCHL4a,片段大小为18.69 cM,在德江、贵阳和罗平点对叶绿素含量的贡献率分别为17.6%、10.6%和11.4%,且来自于亲本综3的等位基因起减效作用。
    结论 不同氮素处理下,检测出一些共有的玉米氮响应的主效 QTLs,如qLAI3b (umc1844~umc1320~bnlg1182)、qLN7a (umc1642~umc2160~umc1929)、qLN7b (phi328175)。这些区段可能在玉米氮素吸收、转运和利用过程中起重要作用,可作为下一步精细定位和图位克隆玉米叶片相关基因的重要候选区域。

     

    Abstract:
    Objectives Leaf is the important organ for photosynthesis, transpiration and resistance in crops. The purpose of this study was to provide an important theoretical basis for breeding maize cultivars with high sunlight utilization efficiency, to dissect genetic variation of maize leaf-related traits under normal and no nitrogen application, and to map related QTLs.
    Methods Using maize backbone Zong3 as the donor and Xu 178 as the acceptor, a total of 166 single segment substitution lines (SSSLs) of maize backbone Zong3 were constructed with Xu 178 as the background by means of hybrid, backcrossing and molecular marker assisted selection. All the SSSLs and Xu 178 were treated under normal (N+) and no N supply (N–) conditions in field plot experiment. The experiments were conducted in Guiyang and Dejiang City of Guizhou Province and Luoping City of Yunnan Province. The leaf area (LA), chlorophyll content (CC) and number of green leaves under ear (NGLE) were investigated.
    Results Across the whole genomes, 42 leaf-related traits QTLs were identified under both N+ and N– conditions and located on ten chromosomes. Under N+ treatment, we identified 8 QTLs related to leaf area, 5 QTLs related to green leaf number under ear, and 8 chlorophyll content QTLs at the three experimental sites. qLAI1b was detected in all the three sites, and it contributed 14.41%, 14.47% and 16.38% of phenotypic variation in Dejiang, Guiyang and Luoping, respectively. The allele from Zong3 exaggerated the phenotypic effects. Besides, the QTLs (qLN7a and qLN7b) related to the number of green leaves under ear were detected in the three experimental sites. Under N– treatment, we identified 9 QTLs related to LA, 7 QTLs related to NGLE, and 8 chlorophyll content QTLs at the three experimental sites. Among them, QTL qLAI3b, which was located on bin3.08, whose fragment size was 120.48 cM, and it contributed 20.4% (Dejiang), 12.8% (Guiyang) and 13.2% (Luoping), respectively. The allele from Xu178 synergistic the phenotypic effects. QTL qLN9 (umc1957~umc1867~umc2078), which was located in bin9.01, whose fragments size was 62.7 cM. QTL qCHL4a, which was located on bin4.08, whose fragment size was 18.69 cM, and it contributed 17.6% (Dejiang), 10.6%(Guiyang)and 11.4% (Luoping), respectively. The allele from Zong3 exaggerated the phenotypic effects.
    Conclusions Two QTLs are detected under two nitrogen treatments, one is qLAI3b, which is located on Bin3.01 with flank markers of umc1844~umc1320~bnlg1182, and the other are qLN7a and qLN7b, which are located on bin7.01 and bin7.05 with flank markers of umc1642~umc2160~umc1929 and phi328175. These loci may play important roles in nitrogen absorbing, transporting and utilization during maize development, and served important candidate loci in further map-based cloning of maize leaf-related traits.

     

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