• ISSN 1008-505X
  • CN 11-3996/S
梁媛媛, 李东亚, 许恒, 王祎, 汤继华, 韩燕来. 高低氮条件下玉米SSSL群体生物量、氮浓度及氮累积量的QTL定位[J]. 植物营养与肥料学报, 2020, 26(7): 1226-1238. DOI: 10.11674/zwyf.19456
引用本文: 梁媛媛, 李东亚, 许恒, 王祎, 汤继华, 韩燕来. 高低氮条件下玉米SSSL群体生物量、氮浓度及氮累积量的QTL定位[J]. 植物营养与肥料学报, 2020, 26(7): 1226-1238. DOI: 10.11674/zwyf.19456
LIANG Yuan-yuan, LI Dong-ya, XU Heng, WANG Yi, TANG Ji-hua, HAN Yan-lai. QTL mapping for plant biomass, nitrogen concentration and nitrogen accumulation in maize under high and low N conditions using SSSL[J]. Journal of Plant Nutrition and Fertilizers, 2020, 26(7): 1226-1238. DOI: 10.11674/zwyf.19456
Citation: LIANG Yuan-yuan, LI Dong-ya, XU Heng, WANG Yi, TANG Ji-hua, HAN Yan-lai. QTL mapping for plant biomass, nitrogen concentration and nitrogen accumulation in maize under high and low N conditions using SSSL[J]. Journal of Plant Nutrition and Fertilizers, 2020, 26(7): 1226-1238. DOI: 10.11674/zwyf.19456

高低氮条件下玉米SSSL群体生物量、氮浓度及氮累积量的QTL定位

QTL mapping for plant biomass, nitrogen concentration and nitrogen accumulation in maize under high and low N conditions using SSSL

  • 摘要:
    目的 玉米生物量、氮浓度以及氮累积量与籽粒的产量和品质密切相关,本研究利用单片段代换系群体,对高氮和低氮条件下玉米成熟期的生物量、氮浓度和氮累积量进行了QTL定位,旨在为氮高效相关基因的精细定位以及克隆氮高效相关的主效QTL奠定基础。
    方法 以氮效率具有显著差异的‘许178’和‘综3’为亲本构建的玉米单片段代换系 (SSSL) 群体作为研究材料,设置高氮 (0.15 g/kg) 和低氮 (0.05 g/kg) 两种处理进行盆栽试验。在成熟期取样,测定植株的生物量、氮浓度以及氮累积量。根据代换系与亲本‘许178’表型值的T-test结果, 利用该群体SSR遗传连锁图谱,在P < 0.05条件下定位所测定性状的QTL。
    结果 在高氮和低氮条件下,共定位到133个QTL (贡献率为 –40.75% ~12.69%)。其中包括49个生物量QTL,在高氮条件下检测到26个、低氮条件下检测到23个;24个氮浓度QTL,其中17个茎秆氮浓度QTL (包括8个高氮条件下检测到的QTL和9个低氮条件下检测到的QTL),7个叶片氮浓度QTL (5个高氮条件下检测到的QTL和2个低氮条件下检测到的QTL);60个氮累积量QTL,包括33个茎秆氮累积量QTL (27个高氮条件下检测到的QTL和6个低氮条件下检测到的QTL),27个叶片氮累积量QTL (11个高氮条件下检测到的QTL和16个低氮条件下检测到的QTL)。上述QTL在玉米的10条染色体上均有分布,其中以第4条染色体上检测到的数量最多 (19个),第5条染色体上检测到的数量最少 (6个)。
    结论 本研究定位到的生物量和叶片、茎秆氮累积量高氮特异QTL片段有umc1077 ~umc2350 (bin 10.04)、umc2350 ~bnlg1028 (bin 10.04) ,低氮特异QTL片段有umc2377 ~bnlg1647 (bin 3.01)、end ~phi072 (bin 4.00)、bnlg1444 ~umc2041 (bin 4.08)、bnlg1863 ~bnlg2046 (bin 8.03)。这些染色体片段中极可能包含控制玉米氮效率相关的关键基因,在后期的试验中我们将逐步对这些QTL进行精细定位。

     

    Abstract:
    Objectives Plant biomass, nitrogen concentration and nitrogen accumulation of maize are closely related to grain yield and quality. In this study, we have mapped QTLs of these traits at maturity stage under high and low nitrogen levels by using single segment substitution lines (SSSL). The purpose of this study is to lay a foundation for fine mapping of genes that related to nitrogen efficiency and cloning of major QTLs.
    Methods The SSSL constructed by Xu178 and Zong 3, which had significant difference in nitrogen efficiency, were used for pot culture. High nitrogen (HN, 0.15 g/kg) and low nitrogen (LN, 0.05 g/kg) were setup for all the lines. The plant biomass, nitrogen concentration and nitrogen accumulation of the leaves and stalks of maize were analyzed at mature stage. The T-test method was used to analyze the phenotype differences between SSSL and recurrent parent Xu178, and the QTLs were mapped in the SSR genetic linkage map at P < 0.05.
    Results 133 QTLs (additive effect contribution: from –40.75%~12.69%) were mapped under high and low nitrogen conditions. There were 49 QTLs related to plant biomass, among which 26 QTLs were detected under HN condition and 23 QTLs were detected under LN condition. And 24 QTLs for N concentration were detected, containing 17 QTLs for stalk N concentration (8 QTLs under HN condition and 9 QTLs under LN condition), and 7 QTLs for leaf N concentration were detected (5 QTLs under HN condition and 2 QTLs under LN condition). There were 60 QTLs for N accumulation, including 33 QTLs for stalk N accumulation (27 QTLs under HN and 6 QTLs under LN condition). There were 27 QTLs detected for leaf N accumulation (11 QTLs under HN and 16 QTLs under LN condition). These QTLs were distributed on 10 chromosomes of the maize, and the chromosome 4 was located the most (19), whereas the chromosome 5 located the least (only 6 QTLs).
    Conclusions In this study, the HN-specific QTLs for shoot dry weight and N accumulation in leaf and stalk were mapped on segments of umc1077–umc2350 (bin 10.04), umc2350–bnlg1028 (bin 10.04). And the LN-specific QTLs for them were mapped on segments of umc2377–bnlg1647 (bin 3.01), end–phi072 (bin 4.00), bnlg1444–umc2041 (bin 4.08) and bnlg1863–bnlg2046 (bin 8.03). We recommend high possibility of these QTLs containing the key gene segments controlling the nitrogen efficiency of maize, and will continue to conduct fine location in the future.

     

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