Abstract: A pot culture experiment was conducted in greenhouse. The results showed that the genetic difference of response of sixteen spring soybean cultivars to low-P stress arid P fertilizer were significant. There were correlations between leaf acid phosphotase activity (APA). leaf area of plant in vegetutive growing phase and seed yield, and between APA, leaf area, shoot dry weight and N content and biologycal yield under low-P stress condition, but no correlation between them under P fertilizer application cnodition. It indicated that these charactristicses can be used as indexes of screening low-P tolerant soybean genotypes. The intolerant cultivars to low-P stress was more sensitive to p fertilizer than tolerant ones. The cultivars which has higher yield under low-P and p fertilizer application conditions was also found in the experiment. therefore, it is important to furtherly screen this type of genotypes from soybean germplasm.