Abstract: Field experiment and solution culture were conducted to study the characteristic of selenium accumulation in different type soybean. The results showed that the selenium content and amount of accumulation in plant and grain increased significantly by spraying 0.025%(Na2SeO3) on leaves in three types of soybean, the increase range was summer soybeanautumn soybeanspring soybean in plant and autumn soybeansummer soybeanspring soybean in grain under low Se condition. The differences of Se accumulation in spring soybean varieties were also significantly under high Se condition. Solution culture with different Se concentration, the amount of Se accumulation in plant was various at different growth stage of soybean and the order was mature periodflowing periodseedling period. Application of Se with 1.0mg/L the amount of Se accumulation in spring soybean per plant was highest.10 varieties with high Se accumulation were selected.