低温对缺钼冬小麦幼苗生长的影响 II.对氮代谢的影响
Effect of molybdenum on growth of winter wheat seedlings induced by low temperature II. Effect on nitrogen metabolism
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摘要: 对三叶一心的缺钼冬小麦进行 24h低温处理 (-5～0℃) ,了解其对氮代谢诸方面的影响。结果显示 ,缺钼对植株全氮含量基本无影响 ,但能明显影响氮在植株各部位的分配。常温下 ,供钼显著降低植株地上部全氮含量 ,但根部全氮量明显增加 ;低温条件下 ,供钼与不施钼处理的冬小麦根部全氮量无大差别 ;无论温度如何 ,施钼与否 ,各处理地上部、根中可溶性蛋白含量均无显著差别。常温下 ,施钼显著提高地上部游离氨基酸含量 ,低温处理后 ,施钼和缺钼处理的植株地上部游离氨基酸含量均明显提高 ,且施钼处理显著高于缺钼处理。通过对硝酸还原酶活性的测定证实 ,常温下 ,钼对冬小麦体内硝酸还原酶活性无影响 ;在低温条件下 ,缺钼植株地上部、根中硝酸还原酶活性明显低于施钼植株 ,施钼植株硝酸还原酶活性能保持在较高水平。Abstract: Low temperature ( LT,-5～0℃) has been underway for 24h when winter wheat seedlings are 28 days old The results as followed : Mo deficiency has no obvious effects on N content of whole plant but affects distribution of it significantly Under normal temperature (NT) conditions, N content in shoot of Mo applied decreases significantly, while that in root increases obviously,and N content in root has no distinct difference between Mo deficiency and Mo application under LT condition Soluble protein contents in shoot and root have no relation with Mo and LT Under NT conditions, free amino acid content of shoot is enhanced obviously by Mo;after induced by LT, that of both shoot and root increase significantly, especially shoot Nitrate reductase activity of Mo applied keeps at a relative high level under LT conditions
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