水稻吸氮能力与氮素利用率的QTLs及其基因效应分析
QTLs underlying rice root to uptake NH4-N and NO3-N and rice N use efficiency at seedling stage
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摘要: 利用F2(Palawan(IR42)群体的RFLP标记连锁图 ,对水稻根系NH4-N和NO3-N吸收能力及植株氮素生理利用率进行QTL区间作图分析 ,在第 2和 5染色体上分别测得控制水稻根系NH4 N吸收能力的QTL各 1个。前者表现为显著的加性及部分显性效应 ,后者有显著的加性和显性效应 ;在第 5和 6染色体上测得控制水稻幼苗根系NO3-N的吸收能力的QTL各 1个 ,两者的基因效应主要表现为显性效应 ;在第 12染色体上检出控制稻苗氮素生理利用率的QTL 1个 ,具有显著的加性和显性效应。此外 ,通过表型值在各标记的两纯合基因型间的差异显著性检验 ,发现第4染色体上的标记RG91对水稻幼苗根系NH4-N吸收能力也具有显著加性效应Abstract: A molecular marker linkage map of F2 population consisting of 104 RFLP markers was employed to identify the quantitative trait loci (QTLs), which control the abilities of rice root to uptake NH4-N and NO3-N and the N use efficiency at seedling stage in a hydroponic experiment Two QTLs linked with ability to uptake NH4-N were detected One of them located on chromosome 2 showed additive and partial dominant gene effects and another on chromosome 5 showed additive and dominant gene effects Two QTLs associated with ability to uptake NO3-N were identified One of them located on chromosome 5 and another on chromosome 6 both showed dominant gene effects One QTL located on chromosome 12 was found to underlying N use efficiency, which showed significant additive and dominant gene effects Furthermore, significant difference was found between means of two parental classes at the marker RG91 on chromosome 4 in the ability of root to uptake NH4-N by t test
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