碳酸氢钠浸提有机磷测定方法探讨
Study on the measurment of organic phosphorus in sodium bicarbonate extracts
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摘要: 碳酸氢钠浸提有机磷的消煮过程是否有磷的挥发和转化, 确定是否有必要在消煮前添加 饱和 MgCl2 溶液和消煮后水解, 试验测定了焦磷酸与偏磷酸、已知浸提有机磷化合物(核酸与苯 磷酸钠)、两种浸提液(土壤与秸秆)及含葡萄糖的磷酸二氢钾溶液消煮和水解后的无机磷量。 结果表明, 消煮能促进偏磷酸和焦磷酸的水解, 消煮过程没有磷的挥发和转化, 没有磷损失, 消 煮前无需添加饱和 MgCl2 溶液, 消煮后也不用水解。Abstract: Whether there were losses of P during digestion by volatilisation of o rthophosphate o r formation of metaphosphate and pyrophosphate w as studied .The necessity of hydrolysis and additon of saturated M gCl2 w as discussed .The quantities of inorg anic phospho rus after diges- tion and hy drolysis of py rophosphate and metaphosphate , o rganic phosphate , tw o extracts and inorg anic phosphate containing glucose w ere measured .The results show ed that hydroly sis of pyrophosphate and metaphosphate was enhanced during digestion .There were no P losses dur- ing digestion .It' s not necessary to add saturated M gCl2 before digestion and hy drolyze after di- gestion.