Abstract: The study presented the effects of two atmospheric CO2 concentrations (350 and 700 mol/mol), two levels of soil moisture (wet and drought) and five rates of nitrogen fertilizer ( N 0,50,100,150,200 mgkg-1) on soil available nutriet content and nutrient uptake of spring wheat (Triticum aestivum L cv. Dingxi No. 8654). Results showed that high CO2 concentration significantly reduced N uptake of wheat, especially in low N treatment, but had little effect on P and K uptake of wheat. N, P and K uptake by wheat were significantly lower in well-watered treatments compared with in drought treatments. The effects of CO2 increased on soil available N depended on soil water status. In well-watered treatment, soil available N content was lower in the enriched CO2 treatment compared with in the ambient CO2 treatment, while in drought treatment, soil available N content was greater applied with N 50, 100, 150 mgkg-1. High CO2 concentration had little effect on soil available P and K content, but soil available P and K content were significantly greater at low N supply and insufficient water condition than at adequate N and water under adequate P and K supply.