Abstract: The pot experiment was conducted to study the effect of water and nitrogen cooperation on winter wheat yield and quality The results showed that the effect of N fertilizer as inhibited seriously by water stress, only with the condition of water and nitrogen coordination, it was developed abundantly Thereby, N fertilizer could not only increase winter wheat yields but also improve the quality of wheat grains. The elongation stage is the key and sensitive stage, the N fertilizer applied at this stage could not only increase the yield of winter wheat but also increase the content of free amino acid and protein of grain, and improve the quality of grain protein