Abstract: Pot and field experiments were conducted to study the effects of compound bio-organic fertilizer on the growth, yield and quality of flue-cured tobacco. The results indicated that appropriate combined application of bio-organic fertilizer with chemical fertilizer was better than that of application of chemical fertilizer. The effect could not only promote the growth and development of plant, enhance its resistance to disease, but also increase dry matter accumulation and potassium content in leaf, remarkably improve the yield and quality. The economic benefit was significant.