Abstract: The present paper investigated the effect of the uncoupler of oxidative phosphorylation, P-type ATPase (inhibitor), ion channels inhibitor, protein synthesis inhibitor, Fe deficiency, and Zn deficiency on Cu uptake in E. haichowensis and C.communis. The results showed Cu accumulation ability of E. haichowensis was higher than that of C. communis, while the Cu transport ability of E. haichowensis was lower than that of C. communis. Both the (uncoupler) of oxidative phosphorylation DNP and P-type ATPase inhibitor Na_3VO_4 could inhibit Cu uptake, which showed properly initiative Cu uptake in the two plants. Ca~(2+) ion channel inhibitor LaCl_3 inhibited Cu uptake of E. haichowensis, while did not inhibit Cu uptake of C. communis, which demonstrated Cu uptake of E. haichowensis was related to Ca~(2+) ion channel, while Cu uptake of C. communis was not. On the contrary, Cu uptake of C. communis was inhiblited by the protein synthesis inhibitor cycloheximide, while Cu uptake of E. haichowensis was not. Cu uptake in two plants was promoted greatly by Fe deficiency. Cu uptake was hardly affected by Zn deficiency at low Cu, while Cu uptake was promoted at high Cu.