• ISSN 1008-505X
  • CN 11-3996/S
徐开未, 张小平, 陈远学, 陈强, 李登煜, . 钼与花生根瘤菌的复配及在酸性紫色土上的接种效果[J]. 植物营养与肥料学报, 2005, 11(6): 816-821. DOI: 10.11674/zwyf.2005.0618
引用本文: 徐开未, 张小平, 陈远学, 陈强, 李登煜, . 钼与花生根瘤菌的复配及在酸性紫色土上的接种效果[J]. 植物营养与肥料学报, 2005, 11(6): 816-821. DOI: 10.11674/zwyf.2005.0618
XU Kai-wei, ZHANG Xiao-ping, CHEN Yuan-xue, CHEN Qiang, LI Deng-yu, Kristina LindstrOm. Co-inoculation of peanut bradyrhizobium and molybdate and its inoculation effect on acid purple soil[J]. Journal of Plant Nutrition and Fertilizers, 2005, 11(6): 816-821. DOI: 10.11674/zwyf.2005.0618
Citation: XU Kai-wei, ZHANG Xiao-ping, CHEN Yuan-xue, CHEN Qiang, LI Deng-yu, Kristina LindstrOm. Co-inoculation of peanut bradyrhizobium and molybdate and its inoculation effect on acid purple soil[J]. Journal of Plant Nutrition and Fertilizers, 2005, 11(6): 816-821. DOI: 10.11674/zwyf.2005.0618

钼与花生根瘤菌的复配及在酸性紫色土上的接种效果

Co-inoculation of peanut bradyrhizobium and molybdate and its inoculation effect on acid purple soil

  • 摘要: 通过供试菌株Spr4-5、Spr2-9耐钼酸钠、钼酸铵试验表明,根瘤菌耐Mo能力强,耐钼酸铵能力比钼酸钠强,不同菌株耐钼能力不同。在研制的“高效根瘤菌+Mo”的复合菌肥中,用钼酸铵生产的复合菌肥比用钼酸钠的活菌数高,高活菌数持续的时间长;复合菌肥的pH一般随含钼量的增加和贮存时间的延长而升高,但复合钼酸铵菌肥pH增幅较小,复合钼酸钠菌肥pH增幅较大。在贮存的180d内,复合钼酸铵的菌肥活菌数和pH均符合《根瘤菌肥料》质量标准;钼酸钠对菌肥的活菌数和pH影响大,钼酸钠不宜作为钼肥添加到根瘤菌剂中。盆栽试验表明,不同菌株共生固氮的有效性与钼酸铵浓度相关。钼酸铵浓度为0.2%时,供试菌株Spr.4-5共生固氮效果最好,比CK增产73.4%,比传统菌肥增产13.7%,占瘤率为59.7%;钼酸铵浓度为0.3%时,Spr.2-9共生固氮效果最好,比CK增产49%,比传统菌肥增产21.4%,占瘤率为70.3%;其增产效果均达极显著水平。

     

    Abstract: The results indicated that the two tested strains spr 45 and spr 29 could resist certain level of Mo.Ammonium molybdate was more suitable in combination with rhizobia than the natrium molybdate.Different strains had different Mo tolerance.The combined inoculant using ammonium molybdate had more viable rhizobial number than that of using natrium molybdate.The pH value of the combined inoculant rose along with the increase of molybdate concentration,also with prolongation of incubation time.Combining inoculant with natrium molybdate increased pH more significantly than that of using ammonium molybdate.Within 180 day incubation,the pH value and viable number of the combined inoculant with ammonium molybdate measured up the quality requirement of 'Rhizobial Fertilizer Standard'.Natrium molybdate was not suitable to be added into rhizobial inoculant,because of the low viable number and its high effect on pH(value).The pot experiment indicated that different strains symbiotic nitrogen fixation efficiency and the ammonium molybdate concentration were related.Spr 45 symbiotic N fixation efficiency was the best with ammonium molybdabte being 0.2% and the yield increased by 73.4%,13.7% compared with CK and traditional inoculant,respectively,nodule(occupancy) was 59.7%.Spr 29 was more effective when the concentration of ammonium molybdate was 0.3%.The(yield)(increased) by 73.4%,13.7% compared with CK and traditional inoculant,respectively,and nodule occupancy was 70.3%.Both of yield effects reached the significant level.

     

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