Abstract: Ear in Vitro culture technology was employed to study the disciplinarian of the effect of zinc on spring wheat growth at the last stages and yield character.The selected 6 zinc levels were 0,0.3,3,6,30 and 300 mmol/L.The results showed that spring wheat appeared to grow best under Zn2+ concentration of 6 mmol/L,in which the chlorophyll content in flag leaf,length of the first joint under spike,kernel number per spike,grain percentage,1000-grain weight and dry matter weight of spike were significantly or highly significant higher than those of CK;However,the difference of length of the second joint under spike and flowering number was not significant between these two treatments.Growth of wheat was restrained under the condition of both low zinc(the concentration of Zn2+ is 0.3 mmol/L) and high zinc(the concentration of Zn2+ is 300 mmol/L).All the trait index under low zinc condition was lower than those of CK.High zinc application not only reduced yield but also caused the waste of zinc fertilizer and environment pollution.