• ISSN 1008-505X
  • CN 11-3996/S
陈淑芳, 朱月林, 张古文, 刘正鲁, 魏国平. NaCl胁迫对嫁接番茄根系质膜和液泡膜ATP酶活性的影响[J]. 植物营养与肥料学报, 2008, 14(6): 1098-1103. DOI: 10.11674/zwyf.2008.0611
引用本文: 陈淑芳, 朱月林, 张古文, 刘正鲁, 魏国平. NaCl胁迫对嫁接番茄根系质膜和液泡膜ATP酶活性的影响[J]. 植物营养与肥料学报, 2008, 14(6): 1098-1103. DOI: 10.11674/zwyf.2008.0611
CHEN Shu-fang, ZHU Yue-lin, ZHANG Gu-wen, LIU Zheng-lu, WEI Guo-ping. Effects of NaCl stress on ATPase activities of root plasma and tonoplast membranes in grafted tomato seedlings[J]. Journal of Plant Nutrition and Fertilizers, 2008, 14(6): 1098-1103. DOI: 10.11674/zwyf.2008.0611
Citation: CHEN Shu-fang, ZHU Yue-lin, ZHANG Gu-wen, LIU Zheng-lu, WEI Guo-ping. Effects of NaCl stress on ATPase activities of root plasma and tonoplast membranes in grafted tomato seedlings[J]. Journal of Plant Nutrition and Fertilizers, 2008, 14(6): 1098-1103. DOI: 10.11674/zwyf.2008.0611

NaCl胁迫对嫁接番茄根系质膜和液泡膜ATP酶活性的影响

Effects of NaCl stress on ATPase activities of root plasma and tonoplast membranes in grafted tomato seedlings

  • 摘要: 在NaCl胁迫下,对番茄嫁接苗和自根苗的根系活力、根系质膜H+-ATPase、液泡膜H+-ATPase和H+-PPase、质膜和液泡膜Ca2+-ATPase、质膜氧化还原系统活性进行了比较。结果表明,胁迫条件下,嫁接苗根系活力显著高于自根苗。胁迫前期,嫁接苗根系质膜H+-ATPase活性、液泡膜H+-ATPase和H+-PPase活性、质膜和液泡膜Ca2+-ATPase活性、质膜NADH氧化速率和Fe (CN)63- 还原速率被显著诱导;自根苗根系液泡膜H+-ATPase、H+-PPase和Ca2+-ATPase活性、质膜NADH氧化速率和Fe (CN)63- 还原速率被显著诱导。胁迫后期,嫁接苗和自根苗根系各项指标均被显著抑制,但嫁接苗各指标受抑制时间较自根苗晚,且数值上均显著高于自根苗。表明嫁接苗比自根苗具有较强的耐盐性。

     

    Abstract: The effects of NaCl stress on root vigor, H+-ATPase activities of plasma membrane and tonoplast membrane, H+-PPase activities of tonoplast membrane, Ca2+-ATPase activities of plasma membrane and tonoplast membrane, plasma membrane redox system in roots of grafted and own-root tomato (Lycopersicon esculentum Mill.) seedlings were investigated. Results showed that root vigor of grafted seedlings was significantly higher than that of own-root seedlings during the whole stage of stress. In the early stage of stress, H+-ATPase activities of plasma membrane and tonoplast membrane, H+-PPase activities of tonoplast membrane, Ca2+-ATPase activities of plasma membrane and tonoplast membrane, NADH oxidation rate, Fe(CN)63- reduction rate in roots of grafted seedling were significantly increased, H+-ATPase, H+-PPase and Ca2+-ATPase activities of tonoplast membrane, NADH oxidation rate, Fe(CN)63- reduction rate of own-root seedling roots were significantly increased as well. In the late stage of stress, the activities of all indices of both grafted and own-root seedlings were inhibited, but the inhibition of grafted seedlings was later and significantly lighter than those of own-root seedlings during the whole stage of stress. All these results indicated that the tolerance of grafted tomato seedlings to NaCl stress was stronger than that of own-root seedlings.

     

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