• ISSN 1008-505X
  • CN 11-3996/S
刘宗华, 王艳朋, 田国伟, 王春丽, 胡彦民, 汤继华. 施氮和不施氮对玉米子粒品质性状的影响及QTL分析[J]. 植物营养与肥料学报, 2009, 15(4): 778-785. DOI: 10.11674/zwyf.2009.0407
引用本文: 刘宗华, 王艳朋, 田国伟, 王春丽, 胡彦民, 汤继华. 施氮和不施氮对玉米子粒品质性状的影响及QTL分析[J]. 植物营养与肥料学报, 2009, 15(4): 778-785. DOI: 10.11674/zwyf.2009.0407
LIU Zong-hua, WANG Yan-peng, TIAN Guo-wei, WANG Chun-li, HU Yan-min, TANG Ji-hua. Effect and QTL analysis of nutrient traits of maize kernel under N-input and No N-input conditions[J]. Journal of Plant Nutrition and Fertilizers, 2009, 15(4): 778-785. DOI: 10.11674/zwyf.2009.0407
Citation: LIU Zong-hua, WANG Yan-peng, TIAN Guo-wei, WANG Chun-li, HU Yan-min, TANG Ji-hua. Effect and QTL analysis of nutrient traits of maize kernel under N-input and No N-input conditions[J]. Journal of Plant Nutrition and Fertilizers, 2009, 15(4): 778-785. DOI: 10.11674/zwyf.2009.0407

施氮和不施氮对玉米子粒品质性状的影响及QTL分析

Effect and QTL analysis of nutrient traits of maize kernel under N-input and No N-input conditions

  • 摘要: 本研究以玉米杂交种农大108的F2:4、F2:5家系为材料,构建了包含199个SSR标记,覆盖玉米10条染色体的遗传连锁图谱,图谱总长度2101.1 cM,平均间距为10.6 cM。在施氮(+N)和不施氮(-N)两种条件下对群体进行了鉴定,利用近红外反射光谱(NIRS)方法测定了群体子粒的蛋白质、淀粉、油分和赖氨酸含量。结果表明,在不施氮条件下子粒的蛋白质、脂肪和赖氨酸含量下降,而淀粉含量上升,但不同材料的变化幅度不同。采用复合区间作图法共检测到24个与玉米子粒品质性状相关的QTLs,其中施氮条件下13个,不施氮条件下11个,涉及21个不同位点的QTLs,分布在1,2,3,4,7,9染色体上,且集中在第3染色体上的QTLs有6个,第9染色体上的QTLs有7个,分别占测到总QTLs的28.57%和33.33%。单个QTL贡献率在8.05%~34.31%之间,其中,qPro1a是不施氮条件下与蛋白质含量相关的主效QTL,qOil3b是不施氮条件下与油分含量相关的主效QTL,贡献率分别达34.31%和17.66%。在21个不同的QTLs中,9个表现加性效应,8个表现部分显性。

     

    Abstract: n this investigation, two sets of F2:4 and F2:5 families deriving from an elite maize hybrid Nongda108 were used to analyze genetic effects of nutrient components under nitrogen plus (+N) and no-nitrogen plus (-N) conditions in 2004 and 2005. A genetic linkage map was constructed including 199 SSR markers covered 2101.1cM on 10 chromosomes of maize, with an average interval length of 10.6cM. Four nutrient components including protein, starch, oil and lysine in kernel of F2:4 and F2:5 families were measured using near infrared reflectance spectroscopy (NIRS) method. The results show that the contents of protein, oil and lysine in kernel are decreased, while starch content is increased under -N condition, and the changing ranges are dependent on different materials. A total of 24 QTLs(13 for +N and 11 for -N treatment), including 21 different loci, associated with nutrient components of kernel are detected using composite interval mapping method (CIM), locating on chromosome 1, 2, 3, 4, 7 and 9, mainly concentrated on chromosome 3 and 9. The contribution of single QTL to phenotypic variation varies from 8.05% to 34.31%, among them, qPro1a is the main contribution to protein content, accounting for 34.31% and qOil3b is the main contribution to oil content, ccounting for 17.66% under -N condertion. Out of 21 QTLs, nine QTLs appear additive effect, and eight QTLs express partially dominant effect.

     

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