Abstract: Effects of spraying SOD simulation material (SODM), choline chloride (Cc) and diethyl aminoethyl hexanoate (DTA-6) on dry weight, fresh weight and volume of potato roots, and activities of polyphenol oxidase, ascorbic acid oxidase and amylase of potato roots were studied by conducting a field experiment. The potato cultivar is Holand-212 (Solanum tuberosum L.). The results show that great effects are obtained with the DTA-6 treatment, especially for controlling activity of polyphenol oxidase of potato roots. Root dry weight, volume, fresh weight and amylase activity are reduced on the 24th day after the spraying. However, from the 24th day after the spraying, root volume is increased under the SODM treatment, and under the Cc treatment, slight effects on physiological characteristics of potato roots are observed.