Abstract: In order to investigate the effects of grafting cultivation on tomato yield, characteristics of nutrients uptake and utilization, the relationship between cultivation methods and fertilization to tomato yield, NPK uptake and use efficiency was studied using split plot design. The results showed that compared to self-rooted tomato, the yield of grafted tomato was increased by 30.76%, the absorption of N, P2O5 and K2O increased by 27.88%, 28.21% and 24.23%, and the utilization rate of N, P and K increased by 25.67%, 20.41% and 20.97%, respectively. Meanwhile, combined application of N, P and K could increase the yield, absorption and utilization rate of N, P and K compared to single element fertilizer application, the yield of tomato under the treatment of NPK was 42.52%, 68.62% and 50.10% higher compared to that under single application of N, P and K, consequently, the utilization efficiency of N, P and K increased by 103.16%, 181.58% and 93.71%, respectively. In addition, grafting and adding fertilization could enhance the soil N, P and K supply capacity significantly.