• ISSN 1008-505X
  • CN 11-3996/S
段旺军, 杨铁钊, 刘化冰, 张小全, 戴亚, 李东亮. 烟叶氨气补偿点的品种间差异及其与氮素代谢的关系研究[J]. 植物营养与肥料学报, 2011, 17(2): 419-424. DOI: 10.11674/zwyf.2011.9440
引用本文: 段旺军, 杨铁钊, 刘化冰, 张小全, 戴亚, 李东亮. 烟叶氨气补偿点的品种间差异及其与氮素代谢的关系研究[J]. 植物营养与肥料学报, 2011, 17(2): 419-424. DOI: 10.11674/zwyf.2011.9440
DUAN Wang-jun, YANG Tie-zhao, LIU Hua-bing, ZHANG Xiao-quan, DAI Ya, LI Dong-liang. Differences in NH3 compensation point among tobacco (Nicotiana Tabacum L.) cultivars and its relationship with N metabolization[J]. Journal of Plant Nutrition and Fertilizers, 2011, 17(2): 419-424. DOI: 10.11674/zwyf.2011.9440
Citation: DUAN Wang-jun, YANG Tie-zhao, LIU Hua-bing, ZHANG Xiao-quan, DAI Ya, LI Dong-liang. Differences in NH3 compensation point among tobacco (Nicotiana Tabacum L.) cultivars and its relationship with N metabolization[J]. Journal of Plant Nutrition and Fertilizers, 2011, 17(2): 419-424. DOI: 10.11674/zwyf.2011.9440

烟叶氨气补偿点的品种间差异及其与氮素代谢的关系研究

Differences in NH3 compensation point among tobacco (Nicotiana Tabacum L.) cultivars and its relationship with N metabolization

  • 摘要: 为了研究不同成熟特性的烤烟品种叶片氨气补偿点及其差异,利用质外体提取方法对3个烤烟品种NC89、K326和中烟90的叶片质外体铵浓度和pH值进行了测定,并计算不同品种叶片的氨气补偿点(25℃);同时探讨了氨气补偿点与氮素代谢的关系。结果表明,叶片氨气补偿点随着成熟衰老而上升;烟草叶片成熟衰老期氨气补偿点与谷氨酰胺合成酶活性关系密切。衰老速度快、叶片氮素状况(总氮和可溶性蛋白)低的品种谷氨酰胺合成酶活性下降幅度大,氨气补偿点升幅大,绝对值也高。因此,衰老速度快、叶片氮素状况低的品种在叶片衰老期具有更大的氨挥发潜力。品种间氨气补偿点的差异与它们的氮素代谢特性有关。

     

    Abstract: To investigate NH3 compensation point (s) and its differences in leaves of three flue-cured tobacco genotypes and the related N metabolization, foliar apoplastic NH4+ concentration and pH were measured by apoplast extraction technique, the s (25℃) was calculated on the basis of measurements, and the activities of related enzymes and nitrogen parameters were also assayed. In the leaves of the three cultivars, an increase in s was observed as the onset of senescence. Compared with the leaves of slow-senescing cultivar, the leaves of the rapid-senescing tobacco cultivar with lower N status had a stronger decrease in glutamine synthetase activity (GS, EC 6.3.1.2) and a more substantial increase in s. These results indicate that the rapid-senescing cultivar may have a high potential for NH3 emission from senescing leaves into the atmosphere, and the difference in s values is related with the N metabolism of the cultivars.

     

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