Abstract: Through nutrient solution cultivating in greenhouse, the tomato cultivar, Gailiang Maofen 802F1, was selected as the plant material to investigate the effects of 2,4-EBR on anti-oxidizing enzyme system and growth under copper stress. The results show that the exogenous EBR could effectively improve the activities of anti-oxidizing enzyme systems (POD, SOD, CAT, APX) in leaves and root activity under the copper stress, while the MDA content is remarkably reduced. As an effect of a long period, the content of chlorophyll and plant weight are significantly increased. For mineral elements, excessive copper obviously enhances the copper uptake and transplant in tomato plant,