Abstract: In order to screen winter wheat genotype of tolerant low nitrogen tolerance, the physiological indices of winter wheat including plant length, root length, plant dry weight, plant nitrogen accumulation etc. under two nitrogen levels were studied. The absorbion and utilization ability of 15 winter wheat genotypes were evaluated comprehensively by principal components analysis and cluster analysis. The results showed that the 9 single indices could be classified into 4 independent comprehensive components. The quick cluster analysis was used to divide 15 genotypes into three types: Xindong 18, Xindong 15 and Shi 4185 were belonged to lownitrogen tolerate type; Shijiazhuang 8, Xindong 7, Yanzhan 4110, Henong 9901, Han 5316 and Xindong 28 were belonged to median type; Xindong 3, Xinxiang 9408, Xindong 23, Shishen 6185, Yumai 34 and Zheng 9023 were belonged to low nitrogen sensitivity type. LowN tolerance indices of plant dry weight and plant nitrogen accumulation between these three types were significantly different at the 0.05 level.