Abstract: A summer maize field experiment was carried out in Fuyang of Anhui province to investigate the effects of nitrogen applications on plant nutrient contents, yield and quality of summer maize. The results show that grain yield and most of the nutritional quality indicators are improved with the increase of amounts of nitrogen fertilizer applied, which could appear a contrary change trend or even decreased when nitrogen fertilizer amount reached or exceed a certain level. In conclusion, the grain yield of the treatment A2 whose nitrogen fertilizer rate is 300 kg/ha with a 5∶0∶5 ratio of base and topdressing is highest, and is 10902 kg/ha with an increase by 140.5% compared to the control (CK), and increases by 4.6% to 12.3% compared to the other base under the same nitrogen rate. The contents of nitrogen, phosphorus and potassium in maize grain appear the curve trends with the increase in the amount of nitrogen application rate, and nitrogen fertilization could significantly improve summer corn grain crude protein content. In the same amount of nitrogen fertilizer application, the grain protein content of treatment A2 is the highest, which is one of the most appropriate nitrogen fertilizer applying regimes of maize production in the region.