Abstract: In order to verify the optimum nitrogen application rate and planting denisty for high yield and better quality in wheat production, and provide basis for establishment of rational cultivation management, a wheat cultivar, Zhenmai 168 with red seed coat and strong gluten was used as experiment material. The experiment was conducted to study the effect of nitrogen application rate(N 240, 300 and 360 kg/ha) and planting denisty(135104, 180104, 225104, 270104 and 330104 seedlings/ha) on grain yield, yield components and grain quality of Zhenmai 168 in the years of 2011-2012. The results indicate that the nitrogen level and planting denisty show significant effects on grain yield and yield components. The grain yield is increased with the increase of N level and planting denisty, however, there is no significant difference between N 300 and 360 kg/ha on grain yield. The plant density for the highest grain yield is 270104 seedlings/ha. Increasing N level and planting denisty significantly increase the number of spikes per unit. The effect of the N level on grains per spike and thousand grain weight is not obvious, however, grains per spike and thousand grain weight are decreased with the increase of planting denisty. Increasing the level of N significantly increases grain protein and wet gluten content, and improves the dough rheological properties. Increasing planting denisty decreases wet gluten content and dough softening degree, however, planting denisty does not result in a significant change in grain protein content, dough water absorption, development time and stability time. In this study, as far as the grain yield and quality factors are concerned, the optimal nitrogen application rate and planting denisty for Zhenmai 168 are N 300 kg/ha and 270104 seedlings/ha, respectively.