Abstract: To investigate of influence of ABA on coloration and expression of genes related to anthocyanin synthesis, a 25 mg/L of ABA solution was sprayed on peach before the peach coloring(cultivar, Meixiang). The results show that the foliar application of ABA(25 mg/L) has no significant effects on the fruit weight, mass fraction of soluble solids and total soluble sugar(TSS)-acid ratio in flesh, while can improve coloration and the content of anthocyanin by 39.48%. The ABA treatment decreases L* and h* values much, and increases a* value. In addition, the ABA treatment up-regulates CHS expression and significantly accelerates UFGT, DFR and LDOX expression. These results indicate that ABA might be involved in the regulation of anthocyanin accumulation in peach and the improvement of the level of anthocyanin accumulation in peach.