钙对NaCl胁迫下马铃薯脱毒苗离子吸收、分布的影响

魏翠果; 陈有君; 蒙美莲; 李鑫杰; 宋树慧; 任少勇; 肖强

doi:10.11674/zwyf.2015.0419

钙对NaCl胁迫下马铃薯脱毒苗离子吸收、分布的影响

1.
内蒙古农业大学农学院,内蒙古呼和浩特 010019;
2.
内蒙古农业大学生命科学学院,内蒙古呼和浩特 010019;
3.
&&
4.
内蒙古科右前旗农业技术推广中心,内蒙古兴安盟 137400)

基金项目:

现代农业产业技术体系建设专项资金(CARS-10-P17)资助。

详细信息

作者简介:
魏翠果(1987—),女,河南濮阳人,硕士研究生,主要从事马铃薯栽培生理研究。E-mail:weicuiguo0607@sina.cn

中图分类号: S532;S156.4
计量
- 文章访问数: 4274
- HTML全文浏览量: 514
- PDF下载量: 1282
出版历程
- 收稿日期: 2014-04-01
- 修回日期: 2015-08-03
- 刊出日期: 2015-07-24

Effects of calcium on ion absorption and distribution
of virus-free potato seedlings under NaCl stress

1.
College of Agriculture,Inner Mongolia Agricultural University,Hohhot 010019,China;
2.
College of life,Inner Mongolia Agricultural University,Hohhot 010019,China;
3.
Keyouqianqi,Inner Mongolia Agricultural Technology Promotion Center,Hinggan League 137400,China

摘要

摘要: 【目的】马铃薯是对盐分较敏感的农作物,土壤盐渍化会严重影响马铃薯的生长发育及其产量和品质。有关钙对NaCl胁迫下马铃薯离子吸收、分布的研究较少。本文通过研究不同浓度CaCl2对NaCl胁迫下马铃薯脱毒苗离子吸收、分布和运输的影响,探讨钙对NaCl胁迫下马铃薯的调控机制,为盐渍土上马铃薯的生产提供理论依据与技术支持。【方法】以‘克新一号’马铃薯品种为试验材料,采用组织培养方法,将0、5、10、15和20 mmol/L CaCl2与0、25、50和75 mmol/L NaCl分别添加到MS+2mg/L B9+3%蔗糖+0.9%琼脂培养基中,制成不同处理组合的培养基。将继代培养的脱毒苗按单节茎段剪切接种到培养基中进行培养。接种30天时调查脱毒苗生物量和Na+、Cl-、K+、Ca2+、Mg2+、P积累量,并分析Na+/K+、Na+/Ca2+、Na+/Mg2+比值及根系与茎叶的SK、Na、SMg、Na和SCa、Na值,探讨离子吸收、运输及分布情况。【结果】NaCl胁迫抑制马铃薯脱毒苗的生长,随NaCl胁迫浓度的增加,马铃薯脱毒苗鲜重、干重显著下降,各器官Na+和Cl-含量极显著增加,K+含量显著下降,Ca2+ 和Mg2+含量减少,茎、叶中P含量降低而根中P含量增加。Na+/K+、Na+/Ca2+、Na+/Mg2+比值随NaCl胁迫浓度的增加而升高。随NaCl胁迫浓度的增加,马铃薯脱毒苗根系与茎叶的SK、Na和SMg、Na值逐渐降低,SCa、Na值呈先升高后降低趋势。0、25和50 mmol/L NaCl胁迫浓度下,以10 mmol/L CaCl2处理的马铃薯脱毒苗根、茎叶鲜重和干重最高,75 mmol/L NaCl胁迫下以15 mmol/L CaCl2处理的马铃薯脱毒苗生物量最高。各NaCl胁迫浓度下,添加CaCl2后,马铃薯脱毒苗各器官Na+含量明显降低,Cl-含量显著增加,K+、Ca2+、Mg2+含量升高,P含量先降低后升高。0、25、50 和75 mmol/L NaCl胁迫浓度下,添加适量CaCl2可明显降低马铃薯脱毒苗各器官Na+/K+、Na+/Ca2+、Na+/Mg2+比值,提高SK、Na、SMg、Na和SCa、Na值,增强K+、Ca2+、Mg2+向地上部的选择运输能力,抑制Na+向地上部的选择运输能力,维持细胞内离子平衡,缓解盐胁迫造成的营养亏缺。【结论】NaCl胁迫下添加外源钙,能够有效改善马铃薯脱毒苗体内的离子平衡,促进营养吸收,Na+向叶片选择运输能力降低,K+、Ca2+、Mg2+向地上部的选择运输能力增强,离子在各器官水平上的区域化分布发生改变是钙缓解盐胁迫的重要生理机制之一。
- 马铃薯 /
- 钙 /
- NaCl胁迫 /
- 离子吸收分布
Abstract: 【Objectives】Soil salinization seriously affects the growth and development of potato, decreases its yield and quality. In this paper, the influence of different concentrations of CaCl2 on the ion absorption, distribution and transportation of virus-free potato seedlings under the stress of NaCl was studied to explore the regulating mechanism of calcium against the NaCl stress of potato,providing a theoretical basis and technical support for the potato production in saline soil.【Methods】 Tissue culture method was used with ‘Kexin No.1’ as tested potato cultivar. The medium of MS+2mg/L B9+3% sucrose+0.9% agar was chosen and 0,5, 10, 15 and 20 mmol/L CaCl2 and 0, 25, 50 and 75 mmol/L NaCl were added to make treatment mediums. The subcultured virus-free seedlings were cut into single stem segments and inoculated 30 days. The biomass and Na+, Cl-, K+, Ca2+, Mg2+, P accumulation of the virus-free seedlings were measured, and the ratios of Na+/K+, Na+/Ca2+, and Na+/Mg2+ and the SK、Na, SMg、Na and SCa、Na values in root and stem were calculated. 【Results】 The growth of potato seedlings was inhibited under NaCl stress. With the increase of NaCl stress concentration, the fresh and dry weights of seedlings decreased significantly; the Na+ and Cl- contents increased significantly, K+ content decreased significantly, Ca2+ and Mg2+ contents decreased in all the tested parts of seedlings; P contents were reduced in stems and leaves, but increased in roots. Consequently, the ratios of Na+/K+, Na+/Ca2+ and Na+/Mg2+ were increased, the SK、Na, SMg、Na values in roots and stems gradually reduced, and the SCa、Na value increased first and then decreased. Addition of 10 mmol/L of CaCl2 under the NaCl stress of 0, 25, 50 mmol/L, and 15 mmol/L of CaCl2 under 75 mmol/L of NaCl increased the biomass accumulation of potato seedlings obviously. With the addition of CaCl2, the Na+ accumulation in seedlings obviously reduced, Cl- content increased significantly, K+, Ca2+ and Mg2+ contents increased, and P content decreased at first and then increased. Under all the NaCl stress treatments, the addition of suitable amount of CaCl2 could effectively reduce the Na+/K+, Na+/Ca2+, Na+/Mg2+ratios in various organs of seedlings, improve SK、Na, SMg、Na, SCa、Na values, increase the selection of up ground transport of K+, Ca2+ and Mg2+, inhibit that of Na+, maintain intracellular ionic balance and relieve the nutritional deficiency caused by salt stress.【Conclusions】 Adding exogenous calcium under NaCl stress can effectively improve the ion balance of potato virus-free seedlings, promote the absorption of nutrients, reduce the transportation capacity of Na+ to leaves, enhance the selection transport of K+, Ca2+, Mg2+ to aboveground, which is one of the physiological mechanisms for calcium alleviating salt stress.
- potato /
- calcium /
- NaCl stress /
- ion absorption and distribution

HTML全文

参考文献(1)

[null]

[1] 刘国花. 植物抗盐机理研究进展[J]. 安徽农业科学, 2006, 34(23): 6111-6112.
Liu G H. Research progress in mechanism of plant resistance to salt stress[J]. Journal of Anhui Agricultural Sciences, 2006, 34(23): 6111-6112.
[2] 赵自国, 陆静梅. 植物耐盐性研究及发展[J]. 长春师范学院学报, 2002, 21(1): 51-53.
Zhao Z G, Lu J M. Progress or research in plants salt tolerance[J]. Journal of Changchun Normal College, 2002, 21(1): 51-53
[3] Silva J V B, Otoni W C, Martinez A et al. Microtuberization of Andean potato species (Solamun spp.) as affected by salinity[J]. Scientia Horticulturae, 2001, 89(2): 91-101.
[4] Patell R M, Prasher S O, Donnelly D, Bonnell R B. Effect of initial soil salinity and subirrigation water salinity on potato tuber yield and size[J]. Agricultural Water Management. 2001, 46(3): 231-239.
[5] Martinez C A, Maestri M, Lani E G. In vitro salt tolerance and proline accumulation in Andean potato (Solamun spp) differing in frost resistance[J]. Plant Science, 1996, 116(2): 177-184.
[6] Levy D, Tai G C C. Differential response of potatoes (Solanum tuberosum L.) to salinity in an arid environment and field performance of the seed tubers grown with fresh water in the following season[J]. Agricultural Water Management, 2013, 116: 122-127.
[7] 内蒙古自治区土壤普查办公室, 内蒙古自治区土壤肥料工作站. 内蒙古土壤[M]. 北京: 科学出版社, 1994. 489-499.
Soil Survey Office of Inner Mongolia Autonomous Region, Soil and Fertilizer Station of Inner Mongolia Autonomous Region. Inner Mongolia Soil[M]. Beijing: Science Press, 1994. 489-499.
[8] 王芳, 万书波, 孟庆伟, 等. Ca²⁺在植物盐胁迫响应机制中的调控作用[J]. 生命科学研究, 2012, 16(4): 362-367.
Wang F, Wan S B, Meng Q W et al. Regulation of Ca²⁺ in plant response mechanisms under salt stress[J]. Life Science Research, 2012, 16(4): 362-367.
[9] Lauchli A. Calcium, salinity and the plasma membrane [A]. Leonard R T, Hepler P K (ed.). Calcium in plant growth and development[C]. The American Society of Plant Physiologists Symposium Series, 1990, 4. 26-35.
[10] 朱晓军. 钙对盐胁迫下水稻幼苗盐害缓解的效应及机理研究[D]. 南京: 南京农业大学, 2004.
Zhu X J. Mitgative effects and mechanisms of exogenous calcium on rice seedlings under salt stress[D]. Nanjing: Nanjing Agriculture University, 2004.
[11] 杨凤军, 李天来, 臧忠婧, 等. 外源钙施用时期对缓解盐胁迫番茄幼苗伤害的作用[J]. 中国农业科学, 2010, 43(6): 1181-1188.
Yang F J, Li T L, Zang Z J et al. Effects of timing of exogenous calcium application on the alleviation of salt stress in the tomato seedlings[J]. Scientia Agricultura Sinica, 2010, 43(6): 1181-1188.
[12] 王朝霞. 钙、钾营养对NaCl胁迫下马铃薯生长和生理特性的影响[D]. 呼和浩特: 内蒙古农业大学硕士论文, 2011.
Wang Z X. Effects of calcium potassium nutrition on growth and physiological characteristics of potato under NaCl stress[D]. Hohhot: MS Thesis of Inner Mongolia Agricultural University, 2011.
[13] 高俊风. 植物生理学实验指导[M]. 北京: 高等教育出版社, 2006.
Gao J F. Plant physiology experimental guidance[M]. Beijing: Higher Education Press, 2006.
[14] 鲍士旦. 土壤农化分析[M]. 北京: 中国农业出版社, 2008.
Bao S D. Soil and agro-chemistry analysis[M]. Beijing: China Agricultural Press, 2008.
[15] 杨敏生, 李艳华, 梁海永, 等. 盐胁迫下白杨无性系苗木体内离子分配及比较[J]. 生态学报, 2003, 23(2): 271-277.
Yang M S, Li Y H, Liang H Y et al. Ion distribution and comparison in seedlings of white poplar clones under salt stress[J]. Acta Ecologica Sinica, 2003, 23(2): 271-277.
[16] 王清, 黄惠英, 张金文, 等. 外源硅及脯氨酸对盐胁迫下马铃薯试管苗的影响[J]. 中国蔬菜, 2005, (9): 16-18.
Wang Q, Huang H Y, Zhang J W et al. Effect of exogenous silicon and proline on potato plantlet in vitro under salt stress[J]. China Vegetables, 2005, (9): 16-18.
[17] 王新伟, 滕伟丽, 马异泉, 等. 盐胁迫下马铃薯脱毒苗生物产量及Vc含量的变化[J]. 东北农业大学学报, 2000, 31(3): 221-224.
Wang X W, Teng W L, Ma Y Q et al. The difference in biological yields and Vc content of potato virus-free plantlets under sodium stress[J]. Journal of Northeast Agricultural University, 2000, 31(3): 221-224.
[18] Li R L, Shi F C, Fukuda K J et al. Effects of salt and alkali stresses on germination, growth, photosynthesis and ion accumulation in alfalfa (Medicago sativa L.)[J]. Soil Science and Plant Nutrition, 2010, 56: 725-733.
[19] Parida A K, Das A B. Salt tolerance and salinity effects on plants: a review[J]. Ecotoxicology and Environment Safety, 2005, 60: 324-349
[20] Zhu J K. Regulation of ion homeostasis under salt stress[J]. Current Opinion in Plant Biology, 2003, 6: 441-445.
[21] Tavakkoli E, Fatehi F, Coventry S . Additive effects of Na⁺ and Cl^- ions on barley growth under salinity stress[J]. Journal of Experimental Botany, 2011, 62: 2189-2203.
[22] Munns R, Tester M. Mechanisms of salinity tolerance[J]. Annual Review of Plant Biology, 2008, 59: 651-681.
[23] 马艳萍. 不同盐度胁迫对芦荟生长和离子吸收分配的影响[J]. 中国农学通报, 2012, 28(25): 172-178.
Ma Y P. Effects of salt concentration on growth, ion absorption and distribution of Aloe vera L.[J]. Chinese Agricultural Science Bulletin, 2012, 28(25): 172-178.
[24] 韩志平, 郭世荣, 郑瑞娜, 等. 盐胁迫对小型西瓜幼苗体内离子分布的影响[J]. 植物营养与肥料学报, 2013, 19(4): 908-917.
Han Z P, Guo S R, Zheng R N et al. Effect of salinity on distribution of ions in mini-watermelon seedlings[J]. Journal of Plant Nutrition and Fertilizer, 2013, 19(4): 908-917.
[25] 朱义, 何池全, 杜玮, 等. 盐胁迫下外源钙对高羊茅种子萌发和幼苗离子分布的影响[J]. 农业工程学报, 2007, 23(11): 133-137.
Zhu Y, He C Q, Du W et al. Effects of exogenous calcium on the seed germination and seedling ions distribution of Festuca arundinacea under salt stress[J]. Transactions of the Chinese Society of Agricultural Engineering, 2007, 23(11): 133-137.
[26] 门福义, 刘梦芸. 马铃薯栽培生理[M]. 北京: 中国农业出版社, 1995, 178.
Men F Y, Liu M Y. Potato cultivation physiology[M]. Beijing: China Agricultural Press, 1995. 178.
[27] 李先婷. 盐胁迫对豌豆幼苗离子平衡和根际pH变化的影响[D]. 兰州: 兰州大学硕士论文, 2013.
Li X T. Effects of salinity stress on ion balance and rhizosphere pH change of pea (Pisum sativum L.) seedlings [D]. Lanzhou: MS Thesis of Lanzhou University, 2013.
[28] Zhu J K. Genetic analysis of plant salt tolerance using Arabidopsis[J]. Plant Physiology, 2000, 124: 941-948.
[29] 杨小菊, 赵昕, 石勇, 等, 盐胁迫对砂蓝刺头不同器官中离子分布的影响[J]. 草业学报, 2013, 22(4): 116-122.
Yang X J, Zhao X, Shi Y et al. Effects of salt stress on ion distribution in different Echinops gmelini organs[J]. Acta Prataculturae Sinica, 2013, 22(4): 116-122.
[30] 孙孟超, 尹赜鹏, 马晓蕾, 等. 盐胁迫对欧李幼苗生理响应及离子含量的影响[J]. 经济林研究, 2012, 30(2): 33-37.
Sun M C, Yin Z P, Ma X L et al. Effects of salt stress on physiological characteristics and ion contents in cerasus humilis seedlings[J]. Nonwood Forest Research, 2012, 30(2): 33-37.
[31] 柳斌, 周万海, 师尚礼, 等. 外源Ca²⁺和水杨酸对苜蓿幼苗盐害的缓解效应[J]. 中国草地学报, 2011, 01: 42-47.
Liu B, Zhou W H, Shi S L et al. Ameliorating effects of exogenous Ca²⁺ and salicylic acid on alfalfa seedlings under salt stress[J]. Chinese Journal of Grassland, 2011, 01: 42-47.
[32] 郑青松, 王仁雷, 刘友良. 钙对盐胁迫下棉苗离子吸收分配的影响[J]. 植物生理学报, 2001, 27(4): 325-330.
Zheng Q S, Wang R L, Liu Y L. Effects of Ca²⁺ on absorption and distribution of ions in salt-treated cotton seedlings[J]. Plant Physiology Journal, 2001, 27(4): 325-330.
[33] 晏斌, 戴秋杰, 刘晓忠, 等. 钙提高水稻耐盐性的研究[J]. 作物学报, 1995, 21(6): 685-690.
Yan B, Dai Q J, Liu X Z et al. The study on increasing salt resistance of rice by calcium[J]. Acta Agronomic Sinica, 1995, 21(6): 685-690.
[34] Shabala S, Demidchik V, Shabala L et al. Extracellular Ca²⁺ ameliorates NaCl induced K⁺ loss from arabidopsis root and leaf cells by controlling plasma membrane K⁺ permeable channels[J]. Plant Physiology, 2006, 141: 1653-1665.
[35] Chen W C, Cui P J, Sun H Y et al . Comparative effects of salt and alkali stresses on organic acid accumulation and ionic balance of seabuckthorn (Hippophae rhamnoides L.)[J]. Industrial Crops and Products, 2009, 30: 351-358.
[36] 赵长江, 薛盈文, 杨克军, 等. 外源钙对盐胁迫下玉米幼苗不同器官离子含量的影响[J]. 玉米科学, 2012, 20(3): 68-72.
Zhao C J, Xue Y W, Yang K J et al. Effects of exogenous calcium on the ionic contents in different organs of maize seedlings under salt stress[J]. Journal of Maize Sciences, 2012, 20(3): 68-72.
[37] Gnes A, Inal A. Effect of salinity on P induced Zn deficiency in pepper plants[J]. Journal of Agriculture and Forestry, 1999, 23: 459-464.
[38] 李佳霓, 田秀平. 不同玉米品种盐胁迫条件下苗期性状与吸磷量关系的研究[J]. 天津农学院学报, 2013, 20(2): 1-5.
Li J N, Tian X P. Studies on relationship between seedling traits and phosphorus uptake in different maize varieties under salt stress[J]. Journal of Tianjin Agricultural University, 2013, 20(2): 1-5.
[39] 冯固, 李晓林, 张福锁, 李生秀. 盐胁迫下丛枝菌根真菌对玉米水分和养分状况的影响[J]. 应用生态学报, 2000, 11(4): 595-598.
Feng G, Li X L, Zhang F S, Li S X. Effect of AM fungi on water and nutrition status of corn plants under salt stress[J]. Chinese Journal of Applied Ecology, 2000, 11(4): 595-598.
[40] Martinez V, Lauchli A. Phosphorus translocation in salt-stressed cotton[J]. Plant Physiology, 1991, 83: 627-632.

施引文献(10)

期刊类型引用(7)

1.	许世奇，何彦臻，李瑞，户可欣，高铱遥，王旭东. 不同改良剂对盐渍土西瓜耐盐性和生长的影响. 农业环境科学学报. 2023(10): 2301-2312 . 百度学术
2.	袁军伟，李敏敏，刘长江，韩斌，尹勇刚，孙艳，贾楠，郭紫娟，赵胜建. 不同砧木与接穗组合对盐胁迫下的马瑟兰葡萄幼苗离子分布的影响. 土壤通报. 2020(01): 144-151 . 百度学术
3.	李广鲁，王文果，陈志新，胡增辉，冷平生. 钙对盐胁迫下冰叶日中花不同器官离子含量和根部K~+、Na~+吸收的影响. 植物科学学报. 2018(02): 282-290 . 百度学术
4.	耿杰，张琳捷，岳小红，曹靖，代立兰，蔡锐，唐让云. 铵态氮和硝态氮调节盐胁迫豌豆幼苗生长和根系呼吸的作用. 植物营养与肥料学报. 2018(04): 1001-1009 . 本站查看
5.	刘廷武，杨文杰，王凤，蒋红梅，杨钊，徐建明. 肌醇和Ca~(2+)对大白菜幼苗生长发育的协同作用研究. 长江蔬菜. 2017(14): 65-70 . 百度学术
6.	李青，秦玉芝，胡新喜，王万兴，熊兴耀. 马铃薯耐盐性研究进展. 园艺学报. 2017(12): 2408-2424 . 百度学术
7.	欧立军，刘周斌，杨博智，马艳青，张竹青，周书栋，邹学校. 外源Ca~(2+)连续喷施时间对辣椒淹水胁迫的缓解效应研究. 西北植物学报. 2016(10): 2015-2021 . 百度学术