• ISSN 1008-505X
  • CN 11-3996/S
许飞云, 张英娇, 王小云, 孙乐云, 许卫锋. 中度干旱胁迫下内生真菌印度梨形孢对旱稻根系细胞膜H+-ATPase的影响[J]. 植物营养与肥料学报, 2021, 27(4): 675-683. DOI: 10.11674/zwyf.20463
引用本文: 许飞云, 张英娇, 王小云, 孙乐云, 许卫锋. 中度干旱胁迫下内生真菌印度梨形孢对旱稻根系细胞膜H+-ATPase的影响[J]. 植物营养与肥料学报, 2021, 27(4): 675-683. DOI: 10.11674/zwyf.20463
shu
XU Fei-yun, ZHANG Ying-jiao, WANG Xiao-yun, SUN Le-yun, XU Wei-feng. Effects of endophytic fungus Piriformospora indica on root H+-ATPase of upland rice under moderate drought[J]. Journal of Plant Nutrition and Fertilizers, 2021, 27(4): 675-683. DOI: 10.11674/zwyf.20463
Citation: XU Fei-yun, ZHANG Ying-jiao, WANG Xiao-yun, SUN Le-yun, XU Wei-feng. Effects of endophytic fungus Piriformospora indica on root H+-ATPase of upland rice under moderate drought[J]. Journal of Plant Nutrition and Fertilizers, 2021, 27(4): 675-683. DOI: 10.11674/zwyf.20463
shu

中度干旱胁迫下内生真菌印度梨形孢对旱稻根系细胞膜H+-ATPase的影响

Effects of endophytic fungus Piriformospora indica on root H+-ATPase of upland rice under moderate drought

    摘要
  • 摘要:
    目的  研究中度干旱胁迫下，内生真菌印度梨形孢 (Piriformospora indica)对旱稻根系细胞膜H+-ATPase的影响，解析印度梨形孢提高旱稻耐旱性的分子生理机制。
    方法  将无菌旱稻幼苗及带有GFP标签的印度梨形孢菌饼 (P. indica-GFP) 接种于备好的PNM培养基中，于26℃/22℃、14 h /10 h (光/暗) 培养两周，采用激光共聚焦显微镜观察水稻根部定殖的印度梨形孢菌。采用盆栽试验方法，分别种植定殖与未定殖印度梨形孢的旱稻 (Oraza sativa，中旱3) 幼苗，保持土壤中度干旱胁迫，培养幼苗2周后，取根系样品，测定根系细胞膜H+-ATPase及生长情况，生长素IAA含量，以及H+-ATPase相关基因的表达。
    结果  通过旱稻根系转录组分析，发现相比于未接种印度梨形孢的旱稻，接种印度梨形孢旱稻根系生长素相关基因表达显著上调。同时，接种印度梨形孢旱稻根系生长素含量、H+分泌、细胞膜H+-ATPase活性及根毛长度显著高于未接种印度梨形孢旱稻。中度干旱胁迫下，与未接种印度梨形孢旱稻相比，接种印度梨形孢旱稻根系细胞膜H+-ATPase相关基因表达显著上调。此外，中度干旱胁迫下，接种印度梨形孢旱稻地上部干重、根系干重、总根长及根毛长度都显著高于未接种印度梨形孢旱稻。
    结论  在中度干旱胁迫下，印度梨形孢通过提高旱稻根系生长素含量，调控根系细胞膜H+-ATPase活性，进而促进根毛发育。

     

    Abstract:
    Objectives  The study aims to investigate the effects of endophytic fungus Piriformospora indica (P. indica) on root H+-ATPase of upland rice under moderate drought stress. Further, we analyzed the molecular physiological mechanism of P. indica in improving the drought tolerance of upland rice.
    Methods  Plants inoculated with P. indica-GFP were grown under moderately dry soil at 14 h light (26℃)/10 h dark (22℃) conditions. After 2 weeks, the extent of plant inoculation with P. indica-GFP was determined using a confocal laser scanning microscope. We investigated the effects of P. indica on the root plasma membrane (PM) H+-ATPase of upland rice, rice growth, root IAA concentration, and the relative expression of (PM) H+-ATPase genes under moderate drought stress.
    Results  The plants colonized with P. indica showed higher relative expression of auxin-related genes, root IAA concentration, proton release, PM H+-ATPase, and root hair length than the control group. Under moderate drought stress, the plants colonized with P. indica had higher root PM H+-ATPase genes expression than the control group. In addition, shoot dry weight, root dry weight, total root length, and root hair length of colonized plants were significantly higher than those of the control plants under moderate drought stress.
    Conclusions  Our results suggest that P. indica modulates root hair length of upland rice by facilitating root auxin concentration and PM H+-ATPase.

     

    • HTML全文
    • 参考文献(32)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回