• ISSN 1008-505X
  • CN 11-3996/S
殷星, 侯振安, 冶军, 闵伟, 刘凯, 王方斌, 廖欢, 甘浩天, 刘少华, 孙嘉璘. 应用多酚–叶绿素仪监测棉花氮素营养状况研究[J]. 植物营养与肥料学报, 2021, 27(7): 1198-1212. DOI: 10.11674/zwyf.20617
引用本文: 殷星, 侯振安, 冶军, 闵伟, 刘凯, 王方斌, 廖欢, 甘浩天, 刘少华, 孙嘉璘. 应用多酚–叶绿素仪监测棉花氮素营养状况研究[J]. 植物营养与肥料学报, 2021, 27(7): 1198-1212. DOI: 10.11674/zwyf.20617
YIN Xing, HOU Zhen-an, YE Jun, MIN Wei, LIU Kai, WANG Fang-bin, LIAO Huan, GAN Hao-tian, LIU Shao-hua, SUN Jia-lin. Application of polyphenol-chlorophyll meter to monitor cotton N nutrition status[J]. Journal of Plant Nutrition and Fertilizers, 2021, 27(7): 1198-1212. DOI: 10.11674/zwyf.20617
Citation: YIN Xing, HOU Zhen-an, YE Jun, MIN Wei, LIU Kai, WANG Fang-bin, LIAO Huan, GAN Hao-tian, LIU Shao-hua, SUN Jia-lin. Application of polyphenol-chlorophyll meter to monitor cotton N nutrition status[J]. Journal of Plant Nutrition and Fertilizers, 2021, 27(7): 1198-1212. DOI: 10.11674/zwyf.20617

应用多酚–叶绿素仪监测棉花氮素营养状况研究

Application of polyphenol-chlorophyll meter to monitor cotton N nutrition status

  • 摘要:
    目的 通过田间试验,研究使用多酚–叶绿素仪对棉花进行快速无损氮素营养诊断适宜的指标。
    方法 田间试验在新疆石河子市进行,设3个施氮处理,分别为施纯N 0、180和240 kg/hm2,分别用N0、N180和N240表示。所有氮肥分5次随滴灌施入,每次施肥后3天,利用多酚–叶绿素仪 (Dualex-4) 和SPAD叶绿素仪分别测定20株棉花叶片的氮平衡指数 (NBI)、Chl值和SPAD值,同步采样测定棉花叶片全氮含量,及0—20 cm、0—40 cm和0—60 cm土层硝态氮含量。
    结果 随着施氮量的增加棉花叶片全氮含量和土壤硝态氮含量均显著增加。其中,0—40 cm土层硝态氮含量与棉花叶片全氮含量关系最密切。增加氮肥施用量,棉花叶片氮素营养诊断指标NBI、Chl值和SPAD值均显著增加。棉花叶片NBI、Chl和SPAD与叶片全氮含量均呈极显著正相关关系,且相关系数 (r) 均达到0.8以上。相关性模型校验结果表明,棉花叶片全氮含量实测值与预测值的平均相对误差 (RE) 分别为–4.0% (NBI)、–3.1% (Chl) 和–5.7% (SAPD)。其中,氮平衡指数 (NBI) 模型对棉花叶片全氮含量的预测精度最高,与实测值的相关系数达到了0.9143,平均绝对百分比误差 (MAPE) 为6.91%;标准均方根误差 (nRMSE) 为8.21%。棉花叶片NBI、Chl和SPAD与土壤硝态氮的模型决定系数表现为NBI > Chl > SPAD。模型校验分析表明,NBI模型与0—40 cm土层硝态氮实测含量的相关性最高,相关系数为0.9116,预测值与实测值的MAPE和nRMSE分别为14.11%和17.88%。
    结论 应用多酚–叶绿素仪监测棉花氮素营养,氮平衡指数 (NBI) 与棉花叶片氮含量和0—40 cm土层硝态氮含量的相关性最高,预测值与实测值的误差仅为6.91%和14.11%,可以满足膜下滴灌条件下棉花氮素营养的快速诊断需求。

     

    Abstract:
    Objectives We studied the suitable parameter in diagnosing cotton N nutrition using the polyphenol-chlorophyll meter in the field.
    Methods A field experiment was set up in Shihezi, Xinjiang, with three N application rates: N 0, 180, and 240 kg/hm2, expressed as N0, N180 and N240. The N fertilizer treatments were topdressed 5 times with drip irrigation. Nitrogen balance index (NBI), Chl, and SPAD values of 20 cotton plants leaves were measured on the third day after topdressing, using both Polyphenol-chlorophyll meter (Dualex-4) and SPAD chlorophyll meter. The total N content of cotton leaves and the nitrate-nitrogen content in 0–20 cm, 0–40 cm and 0–60 cm soil layers were determined simultaneously.
    Results With increasing N applicationrate, the total N content of cotton leaves and soil nitrate-nitrogen content increased significantly. The soil NO3-N content in the 0–40 cm soil layer had the closest relationship with the total N content of cotton leaves. The NBI, Chl, and SPAD values of cotton leaves significantly increased with increasing N application rate, and were positively correlated with the total N content of cotton leaves. The correlation coefficients were all above 0.8. The average relative error (RE) of the measured and predicted values of leaf total N content was –4.0% (NBI), –3.1% (Chl), and –5.7% (SAPD), respectively. The NBI exhibited the highest prediction accuracy for leaf N content, with an r-value of 0.9143, average absolute percentage error (MAPE) of 6.91%, and the standard root mean square error (nRMSE) was 8.21%. The model determination coefficient (R2) of the diagnostic index with soil NO3-N content was in the descending order NBI > Chl > SPAD. The NBI was the most sensitive to NO3-N content in 0–40 cm soil layer, with r-value of 0.9116, MAPE of 14.11%, and nRMSE of 17.88%.
    Conclusions When using the polyphenol-chlorophyll meter to diagnose N nutrition, we find that the N balance index (NBI) is sensitive and accurate in reflecting cotton leaf N content and the NO3-N content in 0–40 cm soil layer. The average absolute percentage error (MAPE) between the predicted and measured values is as low as 6.91% and 14.11%, respectively, which could meet the requirement for rapid diagnosis of cotton N nutrition.

     

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