• ISSN 1008-505X
  • CN 11-3996/S
李青梅, 王华玲, 张玲玲, 张艳军, 赵建宁, 杨殿林, 王慧. 白三叶草和鼠茅草对果园土壤微生物和线虫群落的影响差异[J]. 植物营养与肥料学报, 2021, 27(6): 1055-1067. DOI: 10.11674/zwyf.20622
引用本文: 李青梅, 王华玲, 张玲玲, 张艳军, 赵建宁, 杨殿林, 王慧. 白三叶草和鼠茅草对果园土壤微生物和线虫群落的影响差异[J]. 植物营养与肥料学报, 2021, 27(6): 1055-1067. DOI: 10.11674/zwyf.20622
LI Qing-mei, WANG Hua-ling, ZHANG Ling-ling, ZHANG Yan-jun, ZHAO Jian-ning, YANG Dian-lin, WANG Hui. Cover cropping with white clover and ratten grass impacts soil microbial and nematode communities[J]. Journal of Plant Nutrition and Fertilizers, 2021, 27(6): 1055-1067. DOI: 10.11674/zwyf.20622
Citation: LI Qing-mei, WANG Hua-ling, ZHANG Ling-ling, ZHANG Yan-jun, ZHAO Jian-ning, YANG Dian-lin, WANG Hui. Cover cropping with white clover and ratten grass impacts soil microbial and nematode communities[J]. Journal of Plant Nutrition and Fertilizers, 2021, 27(6): 1055-1067. DOI: 10.11674/zwyf.20622

白三叶草和鼠茅草对果园土壤微生物和线虫群落的影响差异

Cover cropping with white clover and ratten grass impacts soil microbial and nematode communities

  • 摘要:
    目的 覆盖作物影响果园土壤的微生物和线虫群落,研究不同覆盖作物对土壤微生物和线虫群落的影响特征可为生态果园管理提供理论依据。
    方法 试验于2016年在湖北十堰的猕猴桃园内进行,供试品种为美味猕猴桃 (Actinidia deliciosa),2015年定植。覆盖作物处理为白三叶草、鼠茅草,以清耕为对照 (CK)。连续进行3年试验后,于2019年采集土壤样品,测定土壤水分、全氮、硝态氮和有机碳含量,测定细菌、真菌和线虫数量,并分析微生物、线虫群落结构变化及其影响因素。
    结果 与清耕对照相比,覆盖白三叶草显著提高了土壤含水量、硝态氮、总氮和有机碳含量,降低了土壤C/N值;覆盖鼠茅草显著提高了土壤含水量,但硝态氮、总氮和有机碳含量没有显著变化,且均显著低于白三叶草处理。覆盖白三叶草较清耕显著增加了土壤微生物总量、细菌生物量,而覆盖鼠茅草与清耕没有显著差异。覆盖白三叶草和鼠茅草均显著提高了土壤微生物Shannon-Wiener多样性指数和Simpson优势度指数,但均没有改变土壤的真菌/细菌值和革兰氏阴性菌/革兰氏阳性菌值。土壤中线虫总量、食真菌线虫和植食线虫数量均表现为白三叶草 > 鼠茅草 > 清耕处理,并且白三叶草处理线虫数量显著高于清耕,线虫群落物种丰富度也显著高于清耕。冗余分析 (RDA) 表明,土壤含水量、总氮、有机碳含量是影响土壤微生物、线虫群落的主要环境因子。
    结论 覆盖白三叶草和鼠茅草均影响猕猴桃园土壤的水分、养分含量和生物多样性。与种植鼠茅草相比,种植白三叶草可显著提高土壤碳、氮含量,更有利于增加果园土壤生物多样性,提高线虫数量,形成健康的微生物结构。

     

    Abstract:
    Objectives Cover crop species affect soil microbial and nematode community in orchard soils. We compared the effect of white clover ( Trifolium repens ) and rattan grass ( Vulpia myuros ) to provide theoretical basis for ecological orchard management.
    Methods A field experiment was conducted in a kiwifruit orchard in Shiyan of Hubei Province. The tested kiwifruit was Actinidia deliciosa, which was planted in 2015. Clean tillage was used as control (CK). The soil microbial and nematode community, as well as the physical and chemical properties were analyzed.
    Results Compared with clean tillage, white clover significantly increased soil moisture and NO3-N, total N, soil organic C contents and decreased C/N ratio, improved the total microbial biomass, bacterial biomass; whereas rattan grass only increased soil moisture. Both white clover and rattan grass covers significantly increased the Shannon-Wiener diversity index and Simpson dominance index of microbial community, but not F/B and G+/G– ratio. The number of total nematodes, fungivores and plant parasites in soils were in order of white clover > rattan grass > clean tillage treatment, and the difference between white clover and clean tillage treatment was all significant. Redundancy analysis indicated that soil moisture, total N and organic carbon were the main factors affecting soil microbial and nematode communities.
    Conclusions White clover and rattan grass covering is efficient in keeping soil moisture, nutrient content and microbial community diversity. Different from covering rattan grass, covering white clover could significantly increase soil carbon, nitrogen content, increase the soil microbial biomass and nematode number and richness of its communities. So covering white clover is more efficient for keeping healthy and fertile orchard soil than rattan grass.

     

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