Abstract: Sulfur (S) and nitrogen (N) applicaton to canola (double low oilseed rape) was conducted on S deficient soil in New South Wales, Australia. The results indicated that sulfur supply increased seed yield and oil content significantly, certain amount of N (eg. N80) with low (S10) or without S (S0) might increase seed S concentration but high rate N with high S could depress it. Generally, S concentration in vegetative parts were decreased with plant development. There was a peak of S uptake appearing at stem elongation with S supply (Y-0. 0156X2+0. 0012X+0.5276, R2=0. 804) but exponential decrease (Y=3. 7205X-0.595, (R2=0.935) without S. The S concentration in vegetative parts remained relatively stable during flowering and pod filling stages. There was no significant effect of N supply on vegetative S concentration during earlier growth stages (eg. rosette and stem elongation) but the concentration at middle and late stages (eg. flowering and pod filling) was significantly decreased by N application. Total glucosinolates were decreased by high rate N (N160) but increased by S because of a close correlation between seed S and glucosinolate concentration (Y=1.1572e3.5289x, R2=0.8273). However, even at highest rate of S, the glucosinolate concentration was still far below the standard (40u mol/g in whole seed) so that the oil free meal would not affect feeding quality of animals.