Abstract: Responses of peanut to Nano-Calcium Carbonate were studied through nutrient quartz-sand culture experiment in the greenhouse. In this paper, growth and development and physiological status of peanut was analysis and the effect of Nano-calcium on the uptake of Calcium fertilizer and N, P and K was discussed thoroughly. Luhua No. 4, a dominant peanut variety locally, was selected as the experimental material due to its comparative sensitivity to Calcium fertilizer. Major results were summarized as follows: 1) Applying Nano-CaCO3 (coordinated) with humus acid and organic manures could improved the growth and development of peanut significantly compared with single application of nitric acid calcium. Branches, needles in the dust, leaf area and dry weight of peanut promoted markedly. 2) Applying Nano-CaCO3 (coordinated) with humus acid and organic manures could ameliorate the physiological situation of peanut. Contents of soluble sugar and protein increased notably in the stems and leaves of peanut. 3) Nano-CaCO3 combined humus acid could promote the absorbability of peanut to nutrient elements, such as calcium, nitrogen, phosphorus, and potash, compared with nitric acid calcium. The content of nutrients in shoot and root of peanut increased Ca by 0.72% and 0.32%, N by 1.3% and 0.43%, P by 0.08% and 0.04%, K by 0.49% and 0.01%, respectively.